Immobilization and microencapsulation of Lactobacillus caseii and Lactobacillus plantarum using zeolite base and evaluating their viability in gastroesophageal-intestine simulated condition

Azad Mohammad Hassanzadeh; Mahmoud Sowti Khiabani; Mahshidsadat Sadrnia; Baharak Divband; Omid Rahmanpour; Vahid Jabbari; Pourya Gholizadeh; Hossein Samadi Kafil

doi:10.30827/ars.v58i4.6765

Autores/as

Azad Mohammad Hassanzadeh Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz
Mahmoud Sowti Khiabani Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz
Mahshidsadat Sadrnia Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz
Baharak Divband Drug Applied Research Center, University of Medical Sciences, Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz
Omid Rahmanpour Department of Chemical Engineering, Petroleum University of Technology, Ahwaz
Vahid Jabbari Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz
Pourya Gholizadeh Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz,
Hossein Samadi Kafil Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz

DOI:

https://doi.org/10.30827/ars.v58i4.6765

Palabras clave:

Probiótico, Lactobacillus caseii, Lactobacillus plantarum, zeolita, Micro encapsulación

Resumen

Objetivos: Para mejorar la supervivencia en condiciones gastrointestinales de las bacterias probióticas; hemos desarrollado un nuevo método de encapsulación de probióticos a base de zeolita y almidón de zeolita de alginato de calcio como método eficaz para la administración más segura de probióticos.

Material y métodos: se utilizaron Lactobacillus casei (ATCC 39392) y Lactobacillus plantarum (ATCC 29521) como probióticos. Después de la inmovilización del microorganismo en la base de zeolita; la encapsulación se realizó mediante zeolita-almidón de alginato de calcio con el método de extrusión. Posteriormente; se incubaron en la condición gástrica e intestinal simulada. La viabilidad de estas bacterias se calculó sobre la base del tiempo requerido para reducir la base logarítmica en la población microbiana. Las técnicas SEM se usaron para estudiar la apariencia de las cápsulas.

Resultados: después de la incubación durante 120 minutos bajo condición gástrica simulada y 180 minutos en condiciones de jugo intestinal (pH = 8;2); el recuento de células vivas de L. casei y L. plantarum fue de 6;3 log ufc / ml y de 7;3 log ufc / ml. Sin embargo; para controlar las células; este valor era cero y 2;1 log cfu / ml respectivamente.

Conclusión: De acuerdo con los resultados; tuvimos un aumento de la tasa de supervivencia de L. casei y L. plantarum en condiciones simuladas de gastroesofágico-intestino. Estos resultados indicaron buena eficacia y alto rendimiento de zeolita para inmovilización y microencapsulación de probióticos.

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