Marine Actinomycete Streptomyces rubrogriseus Derived Bioactives are Effective Against Clinical Strains of MRSA

Ambily Balakrishnan; Nevin Kottayath Govindan; V P Limna Mol; Hariharan Sini

doi:10.30827/ars.v65i4.31089

Autores/as

Ambily Balakrishnan Faculty of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies
Nevin Kottayath Govindan Kerala University of Fisheries and Ocean Studies https://orcid.org/0000-0002-0844-9342
V P Limna Mol Faculty of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies
Hariharan Sini Department of Biochemistry, Government College Kariavattom https://orcid.org/0000-0002-0844-9342

DOI:

https://doi.org/10.30827/ars.v65i4.31089

Palabras clave:

Staphylococcus aureus, Streptomyces rubrogriseus, SARM, actinomicetos marinos, Compuestos bioactivos

Resumen

Introducción: Las comunidades microbianas naturales interactúan para desarrollar relaciones mutualistas creando un entorno competitivo que estimula la producción de metabolitos secundarios que pueden desarrollarse como un fármaco potencial contra bacterias resistentes a los medicamentos. La investigación actual profundiza en la posibilidad de cocultivar actinomicetos marinos para elevar la producción de nuevos compuestos bioactivos con actividad antimicrobiana mejorada contra Staphylococcus aureus resistente a la meticilina (SARM).

Método: Se aisló actinomicito de sedimentos marinos, identificado como Streptomyces rubrogresius, se cocultivó con S. aureus. Se extrajeron los compuestos bioactivos y se evaluó su actividad contra variantes de SARM. Los extractos que exhibieron actividad significativa se caracterizaron adicionalmente utilizando Cromatrogafía de gases/espectrometría de masas (GC-MS).

Resultados: El estudio demostró un aumento significativo en la producción de compuestos bioactivos en cocultivo en comparación con el monocultivo. Cuando se probaron contra cepas de SARM, las zonas de inhibición obtenidas a partir de extractos de cocultivo en acetato de etilo (40 mm) revelaron diferencias sustanciales en comparación con las de los monocultivos (CIM: 10 μg/mL). El análisis GC-MS identificó composiciones químicas únicas y posibles resultados sinérgicos en el cocultivo en lugar del monocultivo.

Conclusiones: Los hallazgos de este estudio son de suma importancia ya que ayudan en el descubrimiento de nuevos antibióticos eficaces contra SARM.

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