Alginate microspheres of Bacillus subtilis
Keywords:
Alginato, Bacillus subtilis, Inmovilización, MicroesferasAbstract
La microencapsulación de organismos ha sido considerada como una alternativa de inmovilización de células, a finde que éstas puedan ejercer sus funciones en forma gradual. El objetivo del presente estudio fue elaborar microesferasde Bacillus subtilis ya sea en forma esporulada como vegetativa.Microesferas de Bacillus subtilis son preparadas utilizando alginato de sodio. Algunas propiedades típicas del sistemamicroencapsulado, tales como contenido de microorganismos, tamaño de partícula y tiempo de germinación han sidoestudiados. Las microesferas se prepararon mediante el método de coaservación-separación de fases, utilizando unaetapa intermedia de emulsión múltiple. Las condiciones de preparación han sido lo suficientemente benignas parano producir cambios en las propiedades biológicas generales del sistema, pero con la protección que le otorga lamatriz del hidrogel, la cual evita la directa comunicación con el medio externo.La viabilidad demostrada por las microesferas con las formas esporuladas fue significativamente superior a las delas formas vegetativas.Downloads
References
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