Silico identification, molecular characterization and expression analysis of the Trypanosoma brucei paraflagellar rod protein PFR3

Authors

  • M MORELL Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina «López Neyra», CSIC. Granada
  • JL GARCÍA-PÉREZ Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina «López Neyra», CSIC. Granada
  • MC THOMAS Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina «López Neyra», CSIC. Granada
  • MC LÓPEZ Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina «López Neyra», CSIC. Granada

Keywords:

Copy number, Gene, Leishmania, Molecular characterization, Paraflagellar rod protein, Silico identification, Trypanosoma brucei, Trypanosoma cruzi, Transcription

Abstract

In the present paper we describe the identification and isolation of the gene coding for T. brucei PFR3protein. The deduced amino acid sequence produces a protein of 592 residues with an isoelectric pointof 5.14 and shows a 68.9% sequence identity with T. cruzi PFR3 protein. However, the percentage ofhomology among T. brucei PFR3 and other available PFRs sequences from T. brucei and T. cruzi islower than 22%. In contrast to that described for members of paraflagellar rod protein family, thehighest divergence between T. cruzi and T. brucei PFR3 proteins is located at the central region of theprotein with a 38% of similarity over 200 amino acid. We estimate that there exist two copies of theT. brucei PFR3 protein per haploid genome. The gene is transcribed as a mRNA of approximately 3.6kb in length, equally abundant in both procyclic and bloodstream parasite forms.

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Published

2004-12-20

How to Cite

1.
MORELL M, GARCÍA-PÉREZ J, THOMAS M, LÓPEZ M. Silico identification, molecular characterization and expression analysis of the Trypanosoma brucei paraflagellar rod protein PFR3. Ars Pharm [Internet]. 2004 Dec. 20 [cited 2024 May 21];46(1):73-84. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5065

Issue

Vol. 46 No. 1 (2005)

Section

Original Articles

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