Use of two supports for inmobilizing papain

Authors

  • EAR BIASUTTI Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil
  • LM DE MARCO Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil
  • WO AFONSO Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil
  • VDM SILVA Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil
  • DCF LOPES Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil
  • MPC SILVESTRE Depto. de Alimentos/Fac. de Farmácia/UFMG- sala 3070-B3, Av. Antônio Carlos 6627- cep. 31270-901 – Belo Horizonte, MG, Brasil

Keywords:

Activated carbon, Alumina, Contact time, Operational stability, Papain immobilization, Temperature

Abstract

Papain was immobilized on activated carbon (AC) and on alumina (AL), with the aim of preparing low cost dietarysupplements, using whey as hydrolysed protein source. The quantifi cation of the non-adsorbed enzyme, using Lowry’smethod was used to determine the immobilization rate. The effect of the contact time and the temperature was tested,and 30 min at 250C was considered the best condition for immobilizing papain in both supports. AC showed muchhigher immobilization rates (from 95% to 99%) than AL (from 4% to 13%). The reusability of papain was evaluated bymeasuring the residual activity of the enzyme after it has been used for up to 20 times. The quantifi cation of exposurerate of phenylalanine by second derivative spectrophotometry was used to determine the enzyme activity. In this case, ALshowed better results than AC, since the activity of papain remained unchanged after 15 and 5 times, respectively.

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Published

2006-12-20

How to Cite

1.
BIASUTTI E, DE MARCO L, AFONSO W, SILVA V, LOPES D, SILVESTRE M. Use of two supports for inmobilizing papain. Ars Pharm [Internet]. 2006 Dec. 20 [cited 2024 May 18];47(4):425-3. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5043

Issue

Vol. 47 No. 4 (2006)

Section

Original Articles

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