Toxicological assessments of aqueous extracts from seaweeds Bryothamnion triquetrum (Gmelin) M.A.Howe in vitro and models animal studies

Authors

  • A Vidal-Novoa Grupo de Farmacología y Toxicología, Facultad de Biología, Universidad de La Habana (Cuba)
  • A Fallarero-Linares Department of Biochemistry and Pharmacy, Åbo Akademi University, BioCity, (Finland)
  • M Labañino Grupo de Farmacología y Toxicología, Facultad de Biología, Universidad de La Habana (Cuba)
  • A Sánchez-Lamar Grupo de Farmacología y Toxicología, Facultad de Biología, Universidad de La Habana (Cuba)
  • AE Batista-Gonzalez Grupo de Farmacología y Toxicología, Facultad de Biología, Universidad de La Habana (Cuba)
  • AMO Silva Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, USP, São Paulo (Brasil)
  • J Mancini-Filho Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, USP, São Paulo (Brasil)

Keywords:

Toxicological evaluation, Seaweeds, Bryothamnion triquetrum

Abstract

Aim: The aim of this work was to evaluate the toxicity of an aqueous extract from seaweed Bryothamnion triquetrum.

Materials and Methods: Ames assay was developed with S. typhimurium TA 1535, TA 1537 and TA 1538 with and without metabolic activation. Citotoxicity study was carried out with intestinal cells Caco-2 during 24 and 48 hours of exhibition to the extract and the viability was evaluated with the technique of Propidium iodide. Acute Toxicity was carried out with mice Balc/c males for via oral and intraperitoneal and the Toxicity for Repeated Dose was developed with rats Wistar of both sexes, during 3 months for via oral with dose of 8 and 32 mg/kg.

Results: Results of Ames assays showed that this extract is not direct mutagen or promutagen in quantity until 1000 μg. The cytotoxic effect (LC50) of Caco-2 cells after 24 and 48 h of exposition were 9,3 and 4,5 mg/mL respectively. The LD50 of the extract, with intraperitoneal administration was 1205 mg/kg and by oral via not produce mortality in doses until 2000 mg/kg. At the doses of 8 and 32 mg/kg of extract, the repeated oral administration produced no toxic effects.

Conclusions: In summary, this paper adds convincing evidences in support of innocuous of the aqueous extract of B.triquetrum. Altogether; these results represent another step towards the use of this natural product as phytotherapeutical agent.

Downloads

Download data is not yet available.

References

Fitton JH. Brown marine algae, a survey of therapeutic potentials. Alternative & Complementary Therapies. 2003; 9(1):29-33.

Bocanegra A, Bastida S, Benedi J, Rodenas S, Sanchez-Muniz FJ. Characteristics and nutritional and cardiovascular-health properties of seaweeds. J Med Food. 2009; 12(2):236-58.

Suárez AM. Lista de las macroalgas marinas cubanas. Rev Invest Mar. 2005; 26(2):93-148.

Fernández EL, Valiente OG, García R, Velez H, Machytka D, Zsoldos-Mady V, et al. A 1H- and 13C-n.m.r. study of an agar polysaccharide from Bryothamnion triquetrum. Carbohydrate Research. 1987; 163(1):143-7.

Areces AJ. Mariculture of agarophytes in Cuba: present status, trends and perspectives. In: de Oliveira EC, Kautsky N, editores. Cultivation of seaweeds in Latin America.[s.l.] Universidad de Sao Paulo; 1990; 141.

Viana GSB, Freitas ALP, Lima MML, Vieira LAP, Andrade MCH, Benavides NMB. Antinociceptive activity of sulfated carbohydrates from the red algae Bryothamnion seaforthii (Turner) Kütz. and B. triquetrum (S.G. Gmel.) M. Howe. Braz J Méd Biol Res. 2002; 35(6):713-22.

Vidal A, Motidome M, Tanae M, Fallarero A, Brandao L, Mancini J, et al. Actividad antioxidante y ácidos fenólicos del alga marina Bryothamnion triquetrum. Rev Farm Bioquim. Univ Sao Paulo. 2001; 37(3):373-82.

Vidal A, Fallarero A, Silva de Andrade-Wartha ER, de Oliveira e Silva AM, de Lima A, Pavan R, et al. Composición química y actividad antioxidante del alga marina roja Bryothamnion triquetrum (S.G.Gmelin) Howe. Braz j pharm sci. 2006; 42(4):589-600.

Fallarero A, Loikkanen JJ, Männistö PT, Castañeda O, Vidal A. Effects of aqueous extracts of Halimeda incrassata (Ellis) Lamouroux and Bryothamnion triquetrum (S.G.Gmelim) Howe on hydrogen peroxide and methyl mercury-induced oxidative stress in GT1-7 mouse hypothalamic immortalized cells. Phytomedicine. 2003; 10(1):39-47.

Fallarero A, Peltoketo A, Loikkanen JJ, Tammela P, Vidal A, Vuorela P. Effects of aqueous extracts of Bryothamnion triquetrum on chemical hypoxia and aglycemia-induced damage in GT1-7 mouse hypothalamic immortalized cells. Phytomedicine. 2006; 13(4):240-5.

Naidu KA, Tewari A, Joshi HV, Viswanath S, Ramesh HP, Rao SV. Evaluation of nutritional quality and food safety of seaweeds of India. J Food Saf. 1993; 13(2):77-90.

Briand X. Societe d´Engrais Composes Mineraux et Amendments. Utilization of algae extract for the preparation of pharmaceutical, cosmetic, food or agricultural compositions. Patente Americana. US5508033. 1995 Feb 14.

Kang JY, Khan MNA, Park NH, Cho JY, Lee MC, Fujii H et al. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacology. 2008; 116(1):187–90.

Zaragozá MC, López D, Sáiz P, Poquet M, Pérez J, Puig-Paralleda P, et al. Toxicity and antioxidant activity in vitro and in vivo of two Fucus vesiculosus extracts. Agric Food Chem. 2008; 56(17):7773-80.

Beppu F, Niwano Y, Tsukui, Hosokawa M, Miyashita K. Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice. J Toxicol Sci. 2009; 34(5):501-10.

Sekita K, Umemura T, Saito M, Ogawa Y, Ueno K, Kaneko T, et al. Fukuronori (Gloiopeltis furcata) extract: 90-day dietary toxicity study in F344 rats. J food hyg soc jpn. 2001; 42(2):96-101.

Gideon PT, Rengasamy R. Toxicological evaluation of fucoidan from Cladosiphon okamuranus. J Med Food. 2008; 11(4):638–42.

Higa T, Kuniyoshi M. Toxins associated with medicinal and edible seaweeds. Toxin Reviews. 2000; 19(2):119-37.

Maron DM, Ames BN. Revised methods for the Salmonella mutagenicity test. Mutat Res. 1983; 113(3-4):173–215.

CENPALAB. Código práctico para el uso de los animales de laboratorio del CENPALAB. La Habana; 1992.

Litchfiled JT, Wilcoxon F. A simplified method of evaluating dose-effect experiment. J Pharmacol Exp Ther. 1949; 96(2):99-113.

McLaughlin J, Lambert D, Padfield PJ, Burt JPH, O’Neill CA. The mycotoxin patulin, modulates tight junctions in Caco-2 cells. Toxicol in vitro. 2009; 23(1):83-9.

Mahfoud R, Maresca M, Garmy N, Fantini J. The Mycotoxin Patulin Alters the Barrier Function of the Intestinal Epithelium: Mechanism of Action of the Toxin and Protective Effects of Glutathione. Toxicol appl pharmacol. 2002; 181(3):209–18.

Aguilar G. Bryothamnion triquetrum: su capacidad citotóxica, genotóxica y antigenotóxica en células CHO [Tesis Licenciado en Ciencias Biológicas]. La Habana: Universidad de la Habana; 2003.

Charles R. Baseline hematology and clinical chemistry values for Charles River Wistar rats (CRL(W)BR) as a function of sex and age. Technical Bulletin. 1982; 1(2):1-4.

Downloads

Published

2012-06-20

How to Cite

1.
Vidal-Novoa A, Fallarero-Linares A, Labañino M, Sánchez-Lamar A, Batista-Gonzalez A, Silva A, Mancini-Filho J. Toxicological assessments of aqueous extracts from seaweeds Bryothamnion triquetrum (Gmelin) M.A.Howe in vitro and models animal studies. Ars Pharm [Internet]. 2012 Jun. 20 [cited 2025 Apr. 2];53(2):15-20. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4666

Issue

Vol. 53 No. 2 (2012)

Section

Original Articles

License

The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:

  1. The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.

b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.

c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).