Microbiological and toxicological studies of Mitracarpus megapotamicus

Authors

  • Mirta Susana Toribio Cátedra de Farmacología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Pampa (Argentina).
  • Andrea Silvina Pombar Cátedra de Farmacología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Pampa (Argentina).
  • Susana Delia Oriani Cátedra de Microbiología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Pampa (Argentina).
  • Ricardo Enrique Toso Cátedra de Farmacología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Pampa (Argentina).
  • Jésica Gabriela Fernández Cátedra de Farmacología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Pampa (Argentina).

Keywords:

Mitracarpus megapotamicus, Antibacterial activity, Toxicity

Abstract

Aim: To evaluate the antimicrobial activity and toxicity of extracts obtained from the plant species Mitracarpus megapotamicus.

Material and Methods: The minimum inhibitory concentration by agar dilution method of the methanolic extract was determined. The agar diffusion method to check the persistence of antimicrobial activity in chloroform and acetone extractions were used. Acute and subacute toxicity assays in mice were carried out using a single dose of 5,000 mg / kg and a daily dose for 14 days of 1,250 mg / kg of hydroalcoholic extract, respectively.

Results: The minimum inhibitory concentration of the methanol extract was 0.48 mg / ml for Streptococcus equi, Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis and 0.96 mg / ml for Escherichia coli. Antimicrobial activity was also expressed in fractions excerpted with acetone and chloroform, against Staphylococcus aureus. The hydroalcoholic extract produced neither functional changes nor histopathological alterations in studies of acute and subacute toxicity in mice.

Conclusion: The results obtained will allow to design compound identification studies and establish future protocols to determine effectiveness in models in vivo.

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Published

2013-12-20

How to Cite

1.
Toribio MS, Pombar AS, Oriani SD, Toso RE, Fernández JG. Microbiological and toxicological studies of Mitracarpus megapotamicus. Ars Pharm [Internet]. 2013 Dec. 20 [cited 2024 Jul. 22];54(4):09-15. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4650

Issue

Vol. 54 No. 4 (2013)

Section

Original Articles

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