Design and development of a facial Serum with Lactobacillus fermentum CECT 5716
DOI:
https://doi.org/10.30827/ars.v64i3.27306Keywords:
Lactobacillus fermentum, Microbiology, Xerosis, Skin physiology/structure, Formulation/stability, SerumAbstract
Introduction: The skin is of all the organs of the body that is directly and constantly exposed to external agents. Hence the importance of providing all the necessary nutrients to keep it healthy, cared for and hydrated. When this does not happen, the skin begins to lose water, becomes dehydrated, and can be seriously injured. With this intention, a moisturizing serum with natural active ingredients has been designed and developed. It has been developed and characterized through various studies. In addition, it includes a strain of the probiotic species Lactobacillus fermentum CECT 5716, as an innovation, to enhance the synergistic effect of the product.
Method: The cited product is characterized by pH, conductivity, and viscosity measurements to test its stability. Besides it is studied to probiotic viability and consumers’ perception of the product’s efficacy.
Results: Data extracted from pH and conductivity measurements assure that the topical application of our serum is safe, as it does the consumers’ perception of the product’s efficacy study, offering it a better appearance in terms of smoothness and luminosity. This research has revealed the serum’s pseudoplastic characteristics, optimal for its purpose. Lastly, it has been seen that probiotic viability decreases with time.
Conclusions: Based on the results we obtained; it can be affirmed that our moisturizing serum is safe and effective in skin hydration, due to the properties of the raw materials incorporated and the competitive mechanism of the probiotics included.
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References
Sfriso R, Egert M, Gempeler M, Voegeli R, Campiche R. Revealing the secret life of skin - with the microbiome you never walk alone. Int J Cosmet Sci. 2020;42(2):116–26. Doi: 10.1111/ics.12594
Moysidis M, Stavrou G, ChevaIoanna A, Deka I A, Tsetis JK, Birba V, Kapoukranidou D, Tsaousi G, Kotzampassi K. The 3-D configuration of excisional skin wound healing after topical probiotic application. Injury Clin Plast Surg. 2022;531385–1393. Doi: 10.1016/j.injury.2022.02.006
Patti JM, Allen BL, McGavin MJ, Höök M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol. 1994;48: 585-617. Doi:10.1146/annurev.mi.48.100194.003101
Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15: 27. Doi: 10.1128/CMR.15.2.167-193.2002
Chopra A, Gupta A. Skin as an immune organ and the site of biomimetic, non-invasive vaccination. Medicine in Novel Technology and Devices. 2022;16: 100196 . Doi:10.1016/j.medntd.2022.100196
Breton L, Jourdain R, Gueniche A, Bureau-Franz I, Fleith M, Malnoe A. Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin. US20090232785A1, 2009. https://patents.google.com/patent/US20090232785/en?oq=COSMETIC+AND%2fOR+DERMATOLOGICAL+COMPOSITION+FOR+PREVENTION+AND%2fOR+TREATMENT+OF+SENSITIVE+OR+DRY+SKIN
Musthaq S, Mazuy A, Jakus J. The microbiome in dermatology. Clin Dermatol. 2018;36(3): 390–8. Doi: 10.1016/j.clindermatol.2018.03.012
Bustamante M, Oomah BD, Oliveira WP, Burgos-Díaz C, Rubilar M, Shene C. Probiotics and prebiotics potential for the care of skin, female urogenital tract, and respiratory tract. Folia Microbiol. 2020;65(2): 245–64. Doi: 10.1007/s12223-019-00759-3
Gueniche A, Benyacoub J, Blum S, Breton L, Castiel I. Probiotics for skin benefits. In: Nutritional Cosmetics. Elsevier; 2009. Doi: 10.1016/B978-0-8155-2029-0.50029-6
Pérez-Sánchez A, Barrajón-Catalán E, Herranz-López M, Micol V. Nutraceuticals for skin care: a comprehensive review of human clinical studies. Nutrients. 2018;24;10(4):403. Doi: 10.3390/nu10040403
Lee HJ, Jeong SE, Lee S, Kim S, Han H, Jeon CO. Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels. Microbiologyopen. 2018;7(2): e00557. Doi:10.1002/mbo3.557
Knackstedt R, Knackstedt T, Gatherwright J. The role of topical probiotics in skin conditions: a systematic review of animal and human studies and implications for future therapies. Exp Dermatol. 2020;29(1): 15–21. Doi: 10.1111/exd.14032
Puebla-Barragan S, Reid G. Probiotics in cosmetic and personal care products: trends and challenges. Molecules. 2021;26;26(5):1249. Doi: 10.3390/molecules26051249
Al-Ghazzewi FH, Tester RF. Impact of prebiotics and probiotics on skin health. Benef Microbes. 2014;1;5(2): 99–107. Doi: 10.3920/BM2013.0040
Jung YO, Jeong H, Cho Y, Lee EO, Jang HW, Kim J, et al. Lysates of a probiotic, Lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model. IJMS. 2019;2;20(17): 4289. Doi: 10.3390/ijms20174289
Lee GR, Maarouf M, Hendricks AJ, Lee DE, Shi VY. Topical probiotics: the unknowns behind their rising popularity. Dermatol Online J. 2019;15;25(5):13030/qt2v83r5wk.
Cinque B, La Torre C, Melchiorre E, Marchesani G, Zoccali G, Palumbo P, et al. Use of Probiotics for Dermal Applications. In: Liong MT, editor. Probiotics. Berlin, Heidelberg: Springer Berlin Heidelberg; 201. Doi: 10.1007/978-3-642-20838-6_9
Pistone D, Meroni G, Panelli S, D’Auria E, Acunzo M, Pasala AR, et al. A Journey on the Skin Microbiome: Pitfalls and Opportunities. IJMS. 2021;12;22(18):9846. Doi:10.3390/ijms22189846
Tavaria FK. Topical use of probiotics: The natural balance: Porto Biomed J. 2017;2(3):69–70. Doi: 10.1016/j.pbj.2017.02.003
Draelos ZD. The science behind skin care: moisturizers. J Cosmet Dermatol. 2018;17(2):138-144. Doi:10.1111/jocd.12490
Szöllősi AG, Gueniche A, Jammayrac O, Szabó-Papp J, Blanchard C, Vasas N, et al. Bifidobacterium longum extract exerts pro-differentiating effects on human epidermal keratinocytes, in vitro. Exp Dermatol. 2017;26(1):92-4. Doi:10.1111/exd.13130
Butler É, Lundqvist C, Axelsson J. Lactobacillus reuteri DSM 17938 as a novel topical cosmetic ingredient: a proof of concept clinical study in adults with atopic dermatitis. Microorganisms. 2020;11;8(7):1026. Doi:10.3390/microorganisms8071026
Goik U, Goik T, Załęska I. The properties and application of argan oil in Cosmetology. Eur J Lipid Sci Technol. 2019;121(4):1800313. Doi:10.1002/ejlt.201800313
Lin TK, Zhong L, Santiago J. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. Int J Mol Sci. 2017;27;19(1):70. Doi:10.3390/ijms19010070
Kiralan M, Yildirim G. Rosehip (Rosa canina L.) Oil. In: Ramadan MF, editor. Fruit Oils: Chemistry and Functionality [Internet]. Cham: Springer International Publishing; 2019. p. 803–14. Doi: 10.1007/978-3-030-12473-1_43
Lochhead R.Y. The Use of Polymers in Cosmetic Products. In: Cosmetic Science and Technology. Elsevier, 2017, 171-221. Doi: 10.1016/B978-0-12-802005-0.00013-6.
American Pharmaceutical Association, Sheskey PJ, editors. Handbook of pharmaceutical excipients. 8. ed. London: Pharmaceutical Press; 2017.
Yu C. The use of corn-derived ingredients in personal care applications. In: Dayan N, editor. Handbook of Formulating Dermal Applications. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2016. Doi: 10.1002/9781119364221.ch5
Abatangelo G, Vindigni V, Avruscio G, Pandis L, Brun P. Hyaluronic acid: redefining its role. Cells. 2020;21;9(7):1743. Doi:10.3390/cells9071743
Fowler J. Understanding the role of Natural Moisturizing Factor in skin hydration. Feature story. 2012 [cited 20 January 2023]. Available in: https://practicaldermatology.com/articles/2012-jul/understanding-the-role-of-natural-moisturizing-factor-in-skin-hydration
Surini S, Nursatyani K, Ramadon D. Gel formulation containing microcapsules of grape seed oil (Vitis vinifera L.) for skin moisturizer. J Young Pharm. 2018;15;10(1):41–7. Doi:10.5530/jyp.2018.10.11
Aslam MN, Lansky EP, Varani J. Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol. 2006;103(3): 311–8. Doi: 10.1016/j.jep.2005.07.027
Aldag C, Nogueira Teixeira D, Leventhal PS. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature. Clin Cosmet Investig Dermatol. 2016; 9: 411–9. Doi:10.2147/CCID.S116158
Ramanunny AK, Wadhwa S, Gulati M, Singh SK, Kapoor B, Dureja H, et al. Nanocarriers for treatment of dermatological diseases: principle, perspective and practices. Eur J Pharmacol. 2021;890:173691. Doi:10.1016/j.ejphar.2020.173691
Thermo Scientific HAAKE RotoVisco 1 [Internet]. Rheology Solutions. Available from: http://www.rheologysolutions.com/thermo-scientific-haake-rotovisco-1/
Thermo Scientific HAAKE RheoWin Software [Internet]. Rheology Solutions. Available from: http://www.rheologysolutions.com/thermo-scientific-haake-rheowin-software/
Conductímetro EC-Metro BASIC 30 [Internet]. Crison Instruments. Available from: http://www.crisoninstruments.com/es/laboratorio/conductimetro/de-sobremesa/ec-metro-basic-30
Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, et al. Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. Molecules. 2013;15;18(11):14161–71. Doi:10.3390/molecules181114161
Bond DM, Morris JM, Nassar N. Study protocol: evaluation of the probiotic Lactobacillus fermentum CECT5716 for the prevention of mastitis in breastfeeding women: a randomised controlled trial. BMC Pregnancy Childbirth. 2017;17(1):148. doi:10.1186/s12884-017-1330-8
Rodríguez-Sojo MJ, Ruiz-Malagón AJ, Rodríguez-Cabezas ME, Gálvez J, Rodríguez-Nogales A. Limos Lactobacillus fermentum CECT5716: mechanisms and therapeutic insights. Nutrients. 2021;21;13(3):1016. Doi: 10.3390/nu13031016
Zhao Y, Hong K, Zhao J, Zhang H, Zhai Q, Chen W. Lactobacillus fermentum and its potential immunomodulatory properties. J Functional Foods. 2019;56: 21–32. Doi: 10.3389/fmicb.2022.1073922
Monografía LACTANZA Hereditum®. 2014. https://www.lactanciasindolor.com/probioticos-mamarios/lactanza-hereditum/
Universitat Politècnica de València E. Modelo experimental para estimar la viscosidad de fluidos no newtonianos: ajuste a expresiones matemáticas convencionales. Ing agua. 2014; 29:18(1):ix.
Adewale FJ, Lucky AP, Oluwabunmi AP, Boluwaji EF. Selecting the most appropriate model for rheological characterization of synthetic based drilling mud. Int J Applied Engin Res. 2017;12(18):16.
Knowlton J, Pearce S. Rheology of cosmetics systems. In: Handbook of Cosmetic Science & Technology. Elsevier; 1993. Doi: 10.1016/B978-1-85617-197-7.50007-1
Gunaseelan K, Umlong IM, Mukhim T, Ismail K. Electrical conductance behavior of Oil-in-Water microemulsions stabilized by Sodium Dodecyl Sulfate and 1-Butanol. Langmuir. 2003;19(18):7276–81. Doi.org/10.1021/la034899k
Maier RM, Pepper IL. Bacterial growth. In: Environmental Microbiology. Elsevier; 2015. Doi: 10.1016/B978-0-12-394626-3.00003-X
Barer MR. Bacterial growth, culturability and viability. In: Molecular Medical Microbiology. Elsevier; 2015. Doi: 10.1016/B978-0-12-397169-2.00010-X
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