Momordica charantia extract modulates inflammatory response in human lymphocytes via suppression of TNF-α
DOI:
https://doi.org/10.30827/ars.v63i4.24256Keywords:
Bitter melon, cytotoxicity, genotoxicity, mutagenicity, inflammatory parametersAbstract
Introducción: Momordica charantia L. es ampliamente utilizada para consumo y medicina tradicional debido a sus actividades biológicas. Sin embargo, se sabe poco sobre los efectos del melón amargo en las células sanas. Por lo tanto, nuestro objetivo fue evaluar los efectos del extracto de Momordica charantia en linfocitos humanos aislados, especialmente en aspectos inflamatorios, citotóxicos, genotóxicos y mutagénicos.
Método: Para ello se preparó un extracto hidroetanólico con frutos y semillas y se procedió a la identificación y cuantificación fitoquímica. Los linfocitos humanos purificados se expusieron a 12,5, 25 y 50 µg/mL de extracto de Momordica charantia durante 24 horas y después de este período.
Resultados: Los datos mostraron que el extracto de Momordica charantia no indujo citotoxicidad, alteraciones en la frecuencia de micronúcleos, ni actividad de interleucina-6, interleucina-10 ciclooxigenasa-2 y producción de óxido nítrico; sin embargo, causó daño en el ADN y una disminución de TNF-α en las condiciones experimentales y células aplicadas.
Conclusiones: Nuestros datos proponen un proceso antiinflamatorio generado por Momordica charantia mediado por la reducción de TNF-α.
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Copyright (c) 2022 Paula Fischer , Gabriela Cristiane Mendes Gomes , Bibiana Frasson Etcheverry , Nathália Vieira Rios , Pamella Eduardha Espindola Chaves , Emily Clori Sotelo , Dyene Nascimento Campos , Luísa Zuravski , Taiane Acunha Escobar , Michel Mansur Machado
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