ffect of cell-to-cell contact on the metabolism of rat cultured astrocytes

Authors

  • L. SÁNCHEZ-ABARCA
  • A. ORFAO
  • A. TABERNERO
  • J.M. MEDINA

Abstract

Striking changes in metabolism are observed due to cell proliferation and differentiaton.

These changes may be partly accounted for by cell cyc1e requirements although differentiaton

relies on metabolic changes. In this work, we attempted to investigate the effect of cellto-

cell contact on lactate utilization as a source of energy and carbon skeletons. After

14 days in primary culture, astrocytes were replated and the rates of lactate oxidation

and lipogenesis were followed over 3d after replating. Under tbis circumstances, tbe

rate of lactate utilization decreased by about 40% as compared to that observed after

14d in primary culture. One day after replating, the rates of lactate oxidation observed

after 14 days in primary culture were regained. However, the rate of lactate oxidation

decreased sharply 3 days after replatig. these results suggest that lactate oxidation is

inhibited when cell-to-cell contact is disrupted.

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References

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MUGNAINI, E.: "Cell junctions of astrocytes, ependyma and related cells in the mammalian central nervous system, with emphasis on the hypothesis of a generalized functional syncytium of supporting cells" In: Astrocytes (1986), Vol. 1, pp. 329-371 , Eds. Fedoroff, S.,Vemadakis, A., Academic Press, Inc., New York.

BINMOLLER, F. J., MULLER, C. M.: "Postnatal-development of dye-coupling among astrocytes in rat visual-cortex. Glia (1992), 6: 127-137.

MEDINA, J. M., VICARIO, C., ruANES, M., FERNÁNDEZ, E.: "Biochernical adaptations to early extrauterine life". In: Perinatal Biochemistry (1992), pp. 233-258, Eds. Herrera, E., Knopp, R., CRC Press, Boca Raton, FL.

VICARIO, C., TABERNERO, A., MEDINA, J. M.: "Regulation of lactate metabolism by albumin in rat neurons and astrocytes from primary culture". Pediatr Res (1993), 34: 709-715 .

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Published

1996-12-20

How to Cite

1.
SÁNCHEZ-ABARCA L, ORFAO A, TABERNERO A, MEDINA J. ffect of cell-to-cell contact on the metabolism of rat cultured astrocytes. Ars Pharm [Internet]. 1996 Dec. 20 [cited 2024 May 11];37(4):719-28. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21279

Issue

Vol. 37 No. 4 (1996)

Section

Original Articles

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