Epitope rnapping of sorne Leishmania infantum antigens for their application in serodiagnostic assays

Authors

  • M. SOTO
  • J.M. REQUENA
  • L. QUIJADA
  • C. ALONSO

Keywords:

Leishmania infantum, Antigens, Serodiagnostic, Acidic ribosomal proteins., histones, peptides

Abstract

In this work we describe tbe characterization offive antigenic proteins of Leishmania infantum, parasite whose infection cause 'canine visceral leishmaniosis in Spain. The following antigenic proteins were cbaracterized: tbe histones H2A and H3, and three members of tbe acidic ribosomal protein family, LiP2a, LiP2b and LiPO. All of tbem were isolated after isolation of tbeir cDNAs by immunoscreening of aL. infantum egtll expression library witb tbe sera from dogs nattirally infected witb tbe parasite. Aminoacid sequence analysis showed that alI of them were conserved relative to the eukaryotic equivalent proteins but also that they possessed divergent regions. Epitope mapping demonstrated that the antigenic determinants were always located in these specific regions of the parasite proteins. In order to develop a serodiagnosis test for canine leishmaniasis these antigenic regions were expressed and purified as independient polypeptides. The usefulness of these recombinant proteins in ELISA assays for canine leishmaniasis diagnosis has been analyzed.

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References

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GÓMEZ, L. C.: Tesis Doctoral (1992), Facultad Veterinaria. Universidad de Extremadura.
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Published

1997-03-20

How to Cite

1.
SOTO M, REQUENA J, QUIJADA L, ALONSO C. Epitope rnapping of sorne Leishmania infantum antigens for their application in serodiagnostic assays. Ars Pharm [Internet]. 1997 Mar. 20 [cited 2024 Jul. 22];38(2-3):273-8. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21161

Issue

Vol. 38 No. 2-3 (1997)

Section

Original Articles

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