Inmovilización y microencapsulación de Lactobacillus caseii y Lactobacillus plantarum usando base de zeolita y evaluando su viabilidad en condiciones simuladas de gastroesofágico-intestino

Azad Mohammad Hassanzadeh, Mahmoud Sowti Khiabani, Mahshidsadat Sadrnia, Baharak Divband, Omid Rahmanpour, Vahid Jabbari, Pourya Gholizadeh, Hossein Samadi Kafil

Resumen


Objetivos: Para mejorar la supervivencia en condiciones gastrointestinales de las bacterias probióticas; hemos desarrollado un nuevo método de encapsulación de probióticos a base de zeolita y almidón de zeolita de alginato de calcio como método eficaz para la administración más segura de probióticos.

Material y métodos: se utilizaron Lactobacillus casei (ATCC 39392) y Lactobacillus plantarum (ATCC 29521) como probióticos. Después de la inmovilización del microorganismo en la base de zeolita; la encapsulación se realizó mediante zeolita-almidón de alginato de calcio con el método de extrusión. Posteriormente; se incubaron en la condición gástrica e intestinal simulada. La viabilidad de estas bacterias se calculó sobre la base del tiempo requerido para reducir la base logarítmica en la población microbiana. Las técnicas SEM se usaron para estudiar la apariencia de las cápsulas.

Resultados: después de la incubación durante 120 minutos bajo condición gástrica simulada y 180 minutos en condiciones de jugo intestinal (pH = 8;2); el recuento de células vivas de L. casei y L. plantarum fue de 6;3 log ufc / ml y de 7;3 log ufc / ml. Sin embargo; para controlar las células; este valor era cero y 2;1 log cfu / ml respectivamente.

Conclusión: De acuerdo con los resultados; tuvimos un aumento de la tasa de supervivencia de L. casei y L. plantarum en condiciones simuladas de gastroesofágico-intestino. Estos resultados indicaron buena eficacia y alto rendimiento de zeolita para inmovilización y microencapsulación de probióticos.


Palabras clave


Probiótico; Lactobacillus caseii; Lactobacillus plantarum; zeolita; Micro encapsulación

Citas


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Fecha de actualización: 11- 03-2018

ISSN: 2340-9894


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