Comparison of Three Independent Methods of Cephalexin Determination by Means of Potassium Caroate
DOI:
https://doi.org/10.30827/ars.v58i2.6380Keywords:
Cephalexin, iodometry, kinetic-spectrophotometry, potassium caroate, voltammetryAbstract
Aims.Three different assays for the quantity determination of Cephalexin in pure substance were developed and compared using potassium caroate as analytical reagent.
Materials and Methods. Cephalexin pure substance was used and analytical reaction by means of KHSO5 as an oxidant was studied. Voltammetric, kinetic-spectrophotometric and iodometric methods were developed and validated.
Results. The chemical reaction is finished with formation of the S-oxide of Cephalexin in the iodometric and voltammetric methods and the S-oxide hydrolysis product in the kinetic-spectrophotometric method. The reaction finishes in 1 min (observation time). The methods have been validated and good results including precision, accuracy and recovery were obtained. The recovery percent ranged from 98.7 to 101.4%, RSD from 1.42 to 3.0%. The methods proposed are linear in a wide range: voltammetry 2-45 µg mL-1, kinetic-spectrophotometry 1-16 µg mL-1, and iodometry 0.05-0.35 mg mL-1. LOD and LOQ were calculated. The kinetic-spectrophotometric method is the most sensitive (LOQ=1.0 µg mL-1).
Conclusions. The methods proposed are rapid, simple and inexpensive and could be applied to pharmaceutical preparation.
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