A practical method for studying the reversible phases of pyocyanin

Authors

  • DAVID A. CONROY Sociedad Anónica de Frigorifico Abglo - Buenos Aires (Argentina)

Abstract

Cultivos de Pseudomonas aeruginosas en agar inclinado a 250 C. Añade a cada tubo cloroformo para disolver el pigmento e introduce tiras de papel Whatman núm. 1 dejando subir por ellas la solución: secado al aire. Introduce la tira en acetona y la deja que ascienda, arrastrando el pigmento azul, el cual cambia al amarillo, pasando por verde azulado y verde amarillento. Comprueba que se trata de Pioxantosa por el color rojo que toman las tiras del papel con a, clorhídrito al 20 %. Método fácil y muy demostrativo.

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References

CONROY, D. A.: «The differential demonstration of plant pigments by a simple method of paper chromatography» forwarded for publication to the Universidad Nacional de La Plata, República Argentina (1959).

WREDE, F.: Z. Hyg. InfectKr., III, 90. (1930) - quoted by Topley and Wilson, «Principies of Bacteriology and Immunity».

FERRAMOLA, R., and MONTEVERDE, J. J.: «Organismos de género Pseudomonras en las aguas del país», Boletín de Obras Sanitarias

de la Nación, 27, pp. 272-285 (1939).

GALE, E. F.: «The chemical activities of bacteria», p. 45, University Tutorial Press, London (1951).

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Published

1960-02-20

How to Cite

1.
CONROY DA. A practical method for studying the reversible phases of pyocyanin. Ars Pharm [Internet]. 1960 Feb. 20 [cited 2024 Jul. 22];1(2):157-61. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5809

Issue

Vol. 1 No. 2 (1960)

Section

Special Articles

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