Identificación de epítopes T citotóxicos restringidos a la molécula HLA-A2.1 en la proteína HSP70 de T. cruzi.
Keywords:
Linfocitos T citotóxicos, HSP70, Trypanosoma cruzi, HLA-A2.1, Ratones transgénicos A2/KbAbstract
El análisis de la capacidad de unión a células T2 realizado con 31 péptidos correspondientes a distintas regiones dela proteína HSP70 de Trypanosoma cruzi, muestra que 14 de estos péptidos tienen una alta o media afinidad por lamolécula presentadora A2.1. Interesantemente, el presente manuscrito pone de manifiesto que la inmunización deratones transgénicos A2/Kb con la proteína recombinante HSP70 de T. cruzi induce CTLs que reconocen células EL4-A2/Kb cargadas de forma independiente con tres de los péptidos con afinidad de unión a moléculas A2. Estospéptidos presentan una homología menor del 65% con sus homólogos de la proteína HSP70 humana. Los resultadosobtenidos permiten sugerir la posibilidad de que la HSP70 de T. cruzi pueda ser usada como diana para induciractividad inmune citotóxica en humanos.Downloads
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