Quantitative HPLC determination of indigotin and indirubin in indigo naturalis

Authors

  • B QIAN Facultad de Ciencias Farmacéuticas, Colegio médico de Kunming, Kunming 650031, República Popular China
  • P PANICHAYUPAKARANANT Facultad de Ciencias Farmacéuticas, Universidad Príncipe de Songkla, Hat-Yai, Songkla 90112, Tailandia
  • A SIRIKATITHAM Facultad de Ciencias Farmacéuticas, Universidad Príncipe de Songkla, Hat-Yai, Songkla 90112, Tailandia
  • RP ZHANG Facultad de Ciencias Farmacéuticas, Colegio médico de Kunming, Kunming 650031, República Popular China
  • YD GUO Facultad de Ciencias Farmacéuticas, Colegio médico de Kunming, Kunming 650031, República Popular China
  • YQ WU Facultad de Ciencias Farmacéuticas, Colegio médico de Kunming, Kunming 650031, República Popular China

Keywords:

Baphicacanthus cusia, HPLC, Indigo naturalis, Indigotin, Indirubin

Abstract

A high-performance liquid chromatographic separation and quantitative method using a RP-18 columneluted isocratically with methanol and water (73:27, v/v) was developed to analyze the isomeric activecompounds, indigotin and indirubin, present in the chloroform extract of indigo naturalis. Standardizedsolutions of indigotin and indirubin were used to evaluate linearity, precision (repeatability), accuracyand specificity of the method were evaluated. The results exhibited a linearity (r2 > 0.999) of between1.8 - 16.2 μg/ml and 1.6 - 14.4 μg/ml for indigotin and indirubin, respectively. A high degree ofspecificity and robustness, as well as repeatability (relative standard deviation less than 5% for bothstandards and the extracts) and accuracy (% recoveries greater than 95%) were also achieved

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Published

2005-09-20

How to Cite

1.
QIAN B, PANICHAYUPAKARANANT P, SIRIKATITHAM A, ZHANG R, GUO Y, WU Y. Quantitative HPLC determination of indigotin and indirubin in indigo naturalis. Ars Pharm [Internet]. 2005 Sep. 20 [cited 2025 Mar. 9];46(4):429-38. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5097

Issue

Vol. 46 No. 4 (2005)

Section

Original Articles

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