A new reverse-phase high performance liquid chromatographic method for determination of flutamide in liposomes, rat blood plasma and tablet dosage forms

Authors

  • ML UMRETHIA Drug Delivery Research Laboratory, Pharmacy Department, The M.S. University of Baroda, Vadodara, India
  • PK GHOSH Drug Delivery Research Laboratory, Pharmacy Department, The M.S. University of Baroda, Vadodara, India
  • RJ MAJITHIYA Drug Delivery Research Laboratory, Pharmacy Department, The M.S. University of Baroda, Vadodara, India
  • RSR MURTHY Drug Delivery Research Laboratory, GH Patel Pharmacy Building, Donor’s Plaza, Opposite to M.S. University Office, Fatehgunj, Vadodara

Keywords:

Flutamide, Reverse-Phase High Performance Liquid Chromatography

Abstract

Flutamide is an anti-androgen widely used in treatment of prostate cancer by affecting Prostate SpecificAntigen (PSA) level in blood, which requires its stability in blood for enough time. A new pharmaceuticaldosage form, liposomes, has been developed for improving efficacy of this drug. The aim of this studyis to develop HPLC UV method for the determination of FLT in liposomes, rat blood plasma and different marketed tablet dosage forms. The method involved less time-consuming sample preparationsteps. The internal standard (IS) for the assay procedure was 6-Mercaptopurine. The samples wereinjected on to reverse-phase (Thermosil® C18) column. The mobile phase, Methanol: Water (80:20, v/v)was run at a flow rate of 1 ml/min for 8 min. The FLT was detected by UV detector at 295 nmwavelength. The retention times for IS and FLT were 3.03 and 4.02 min, respectively. The method waslinear over the concentration range 20-1000 ng/ml and 50-1000 μg/ml in mobile phase and rat bloodplasma, respectively. The method was validated for accuracy, precision, robustness and recovery. Thelimit of detection was 0.099μg/ml and 0.106μg/ml in mobile phase and rat blood plasma, respectively.The method was shown to be highly reproducible and it seems to be adequate for routine therapeuticdrug monitoring. It could be used without any interference from lipids, tablet excipients and endogenoussubstances from the plasma samples.

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Published

2005-03-20

How to Cite

1.
UMRETHIA M, GHOSH P, MAJITHIYA R, MURTHY R. A new reverse-phase high performance liquid chromatographic method for determination of flutamide in liposomes, rat blood plasma and tablet dosage forms. Ars Pharm [Internet]. 2005 Mar. 20 [cited 2024 May 21];46(2):109-24. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5066

Issue

Vol. 46 No. 2 (2005)

Section

Original Articles

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