Procedures to study the in vivo phagocytosis and application for the investigation of immunomodulatory activity of antibiotics
Keywords:
Phagocytosis, Clearance, Mouse model, Extracellular microorganisms, Intracellular microorganisms, Azithromycin, Long-term therapyAbstract
The blood stream clearance of particles is a measure of the functional capacity of the mononuclearphagocytic system, which is responsible for the systemic elimination of pathogenic microorganisms,immunocomplexes and apoptotic cells. This capacity may be altered by biological reponse modifiersresponse,in which numerous antimicrobial agents are present. In this work, the effectiveness of the measurementof clearance capacity was compared in BALB/c mice that were inoculated with different microorganisms(a yeast, two extra and intracellular gram-positive bacteria, and two extra and intracellular gram-negativebacteria). As a means to studying the in vivo modification of phagocytosis by the macrolid antibiotic,azithromycin, the yeast Candida albicans was chosen for its appropriate clearance kinetics and its naturalresistance to antibacterial agents. Treatment with azithromycin for 10 and 20 days reduced clearancecapacity of the mononuclear phagocytic system.Downloads
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