Effect of green tea extract on cisplatin induced oxidative damage on kidney and testes of rats

Authors

  • P LEENA Pharmacy Department, Faculty of Technology and Engineering,The M.S. University of Baroda
  • R BALARAMAN Pharmacy Department, Faculty of Technology and Engineering,The M.S. University of Baroda

Keywords:

Antioxidant, Cisplatin, Free radicals, Green tea, Nephrotoxicity, Testicular toxicity

Abstract

Green tea extract (Camellia sinensis) was administered orally to rats at the dose levels of 25, 50,100mg/kg to investigate its effect on cisplatin (3mg/kg) induced nephrotoxicity and testicular toxicity. Greentea extract restored the level of creatinine, urea, blood urea nitrogen (BUN) and uric acid in serum ofanimals treated with cisplatin as compared to the animals treated with cisplatin alone. It was furtherfound that administration of green tea extract restored the level of antioxidant enzymes such as, superoxidedismutase (SOD), catalase (CAT) and reduced glutathione (GSH), and membrane bound enzymes likeNa+K+ATPase, Ca2+ATPase and Mg2+ATPase and decreased lipid peroxidation (MDA) in kidney and intestes of animals which were altered after chronic treatment with cisplatin. Thus it can be concluded thatgreen tea extract possesses antioxidant activity and by virtue of this action it can protect the kidney andtestes from cisplatin induced oxidative damage.

Downloads

Download data is not yet available.

References

Rosenberg B. Fundamental studies with cisplatin. Cancer1985; 55: 2303–15.

Ward JM, Fauvie KA. The nephrotoxic effects of cis-diam- mine- dichloroplatinum IIin male F344 rats. Toxicol. Appl.

Pharmacol. 1976; 38: 535–547.

Loehrer PJ, Einhorn LH. Drugs five years later. Cisplatin. Ann. Intern. Med. 1984; 100: 704–713.

Somani SM, Ravi, R, Rybak LP. Diethylthiocarbamate protection against cisplatin nephrotoxicity. Drug Chem. Toxicol.

; 18: 151–170.

Husain K, Morris C, Whitworth C, Trammel GL, Rybak LP, Somani SM. 4-Methylthiobenzoic acid protection against

cisplatin nephrotoxicity: antioxidant system. Fundam. Appl. Toxicol. 1996; 32: 278–284.

Seethalakshmi L, Flores C, Kinkead T, Carboni AA, Malhotra RK, Menon M. Effects of subchronic treatment with

cisplatinum on testicular function, fertility, pregnancy outcome, and progeny. J. Androl. 1992;13: 65–74.

Aydiner A, Aytekin Y, Topuz E. Effects of cisplatin on testicular tissue and the Leydig cell–pituitary axis. Oncology 1997;

:74–78.

Hannemann J, Baumann K. Cisplatin-induced lipid peroxidation and decrease of gluconeogenesis in rat kidney cortex:

different effects of antioxidants and radical scavengers. Toxicology 1988; 51: 119–32.

Sadzuka Y, Shoji T, Takino Y. Effect of cisplatin on the activities of enzymes which protect against lipid peroxidation.

Biochem Pharmacol 1992; 43: 1873–5.

Masuda H, Tanaka T, Takahama UT. Cisplatin generates superoxide anion by interaction with DNA in a cell-free system.

Biochem Biophys Res Commun 1994;203:175–80.

Baliga R, Zhang Z, Baliga M, Ueda N, Shah S. In vitro and in vivo evidence suggesting a role for iron in cisplatininduced

nephrotoxicity.Kidney Int 1998; 53: 394– 401.

Matsushima H, Yonemura K, Ohishi K, Hishida A. The role of oxygen free radicals in cisplatin-induced acute renal failure

in rats. J Lab Clin Med 1998; 131:518–26.

Weijl NI, Hopman GD, Wipkink-Bakker A, Lentjes EG, Berger HM, Cleton FJ,Osanto S. Cisplatin combination

chemotherapy induces a fall in plasma antioxidants of cancer patients. Ann Oncol 1998; 9:1331–7.

Yoshida M, Itzuka K, Hara M, Nishijima H, Shimada A, Nakada K, Satoh Y,kama Y, Terada A. Prevention of

nephrotoxicity of cisplatin by repeated oral administration of ebselen in rats. Tohoku J. Exp. Med. 2000;191: 209–220.

Antunes LMG, Darin JDC, Bianchi Mde LP. Protective effect of vitamin C against cisplatin induced nephrotoxicity and

lipid peroxidation in adult rats: a dose dependant study. Pharmacol. Res 2000.; 41: 405– 411.

Caffrey PB, Frenkel GD. Selenium compounds prevent the induction of drug resistance by cisplatin in human ovarian

tumor xenografts in vivo. Cancer Chemother.Pharmacol 2000; 46:74–78.

Antunes LMG, Darin JDC, Bianchi M de LP. Effect of the antioxidants curcumin or selenium on cisplatin induced

nephrotoxicity and lipid peroxidation in rats. Pharmacol. Res. 2001; 43: 145 – 150.

Zhu QY, Zhang A, Tsang D, Huang Y, Chen Z Y. Stability of green tea catechins. J Agric Food Chem1997; 45: 4624–

Harbone JB. General procedures and measurement of total phenolics. In: Harbone J. B., ed. Methods in Plant Biochemistry1989,

Vol. 1. New York:Academic Press, pp. 1–28.

Scott BC, Butler J, Halliwell B, Aruoma O I. Evaluation of the antioxidant action of ferulic acid and catechin. Free Radic

Res Commun 1993; 19: 241–253.

Yokozawa T, Nakagawa T, KyeoungI M, Eun JC, Shigeya T, Terasawa K. Effect of green tea tannin on cisplatin induced

nephropathyin LLC-PK1 cells and rats. J Pharm.Pharmacol 19995;1: 1325-1331.

Lin AM, Chyi BY, Wu LY, Hwang LS, Ho LT. The antioxida-tive property of green tea against iron-induced oxidative

stress in rat brain. Chin. J Physiol 1998;41:189–194.

Mazzio EA, Harris N, Soliman KF. Food constituents attenuate monoamine oxidase activity and peroxide levels in C6

astrocyte cells. Planta Med 1998; 64:603–606.

Hiramoto K, Ojima N,Sako K, Kikugawa K. Effect of plant phenolics on the formation of the spin-adduct of hydroxyl

radical and the DNA strand breaking by hydroxyl radical. Biol. Pharm. Bull. 1996;19: 558–563.

Xu Y, Ho CT, Amin SG, Han C, Chung FL. Inhibition of tobacco-specific nitrosamine-induced lung tumorigenesis in

A/ J mice by green tea and its major polyphenol as antioxidants. Cancer Res. 1992; 52: 3875–3879.

Ahmad N, Mukhtar H. Green tea polyphenols and cancer: biologicmechanisms and practical implications. Nutr. Rev.

; 57: 78–83.

Slater TF, Sawyer BC. The stimulatory effects of carbon tetrachloride and other halogenoalkanes or peroxidative reactions

in liver fractions in vitro. Biochemical Journal 1971;123: 805–814.

Misra H P, Fridovich I.The role of superoxide anion in the autooxidation of epinephrine and a simple assay of SOD.

J Biol.Chem. 1972; 247: 3170-3175.

Colowick SP, Kaplan NO, Packer L. Methods in Enzymology, 1984 Vol. 105 .Academic Press, London, pp. 121–125.

Moron MS, Depierre JW, Mannervik B.Levels of glutathione, glutathione reductase and glutathione S-transferase activities

in rat lung and liver. Biochimica et Biophysica ACTA 1979;582: 67-78.

Bonting SL. Presence of Enzyme System in Mammalian Tissues. Membrane and Ion Transport. Wiley 1970, London,

pp. 257–263.

Hjerten S, Pan H. Purification and characterization of two forms of a low affinity Ca2+ATPase from erythrocyte

membranes. Biochimica et Biophysica ACTA1983;728: 281–288.

Ohnishi T, Suzuki T, Suzuki Y, Ozawa K. A comparative study of plasma membrane Mg2+ATPase activities in normal,

regenerating and malignant cells. Biochimica et Biophysica ACTA1982; 684: 67–74.

Lowry OH, Rosenbrough NJ, Farr AC, Randell RJ. Protein measurement with folin-phenol reagent. J Biol Chem 1975;

: 265–275.

Halliwell B, Gutteridge JMC. Lipid peroxidation: a radical chain reaction. In:Halliwell B., Gutteridge J.M.C.(Eds.), Free

radicals in biology and medicine1999.Clarendon Press,Oxford, UK, pp. 188–276.

Weijl NI, Cleton FJ, Osanto S. Free radicals and antioxidants in chemotherapy –induced toxicity. Cancer Treat.Rev. 1997;

; 209–240.

Kuhlmann MK, Burkhardt G, Köhler H. Insights intopotential cellular mechanisms ofcisplatin nephrotoxicity and their

clinical application. Nephrol. Dial. Transplant.1997; 12: 2478–2480.

Conklin KA. Dietary antioxidants during cancer chemotherapy: impact on chemotherapeutic effectiveness and development

of side effects. Nutr. Cancer 2000; 37:1–18.

Packer L, Landvik S. Vitamin E in biological systems.In: Emerit, I., Packer,L.,Auclair, C. (Eds). Antioxidants in therapy

and preventive medicine 1990. Plenum Press, New York, pp. 93–104.

Zhang JG, Lin dupWE. Cisplatin nephrotoxicity decreases in mitochondrial protein sulfhydryl concentration and calcium

uptake by mitochondria from rat renal cortical slices. Biochem Pharmacol1992; 47: 1127-35.

Kim YK, Jung JS, Lee SH, Kim YW. Effects of antioxidants and calcium in cisplatininduced renal injury in rabbit renal

cortical slices. Toxicol. Appl. Pharmacol. 1997;146: 261-269.

Mukhtar H, Wang ZY, Katlya SK, Agarwal R. Tea components: antimutagenic and anticarcinogenic effects. Preventive

Medicine 1992;21: 351-/360.

Sano M, Takahashi Y, Yoshino K, Shimoi K, Nakamura Y,Tomita I, Oguni I Konomoto H. Effect of tea (Camellia

sinensis L.) on lipid peroxidation in rat liver and kidney: a comparison of green and black tea feeding. Biol Pharma

Bull 1995;18: 1006-1008.

Shim JS, Kang MH, KimYH, Roh JK, Roberts C, Lee IP. Chemopreventive effect of green tea (Camellia sinensis )among

cigarette smokers. Cancer Epidemiol Biomarkers Prev 1995; 4: 387-/391.

Gomes A, Vedasiromoni JR, Das M, Sharma RM, GangulyDK. Anti-hyperglycemic effect of black tea (Camellia sinensis

) in rat. J Ethnopharmacology 1995;45:223-/226.

Yang JA, Choi JH, Rhee SJ. Effects of green tea catechin on phospholipase A2 activity and antithrombus in streptozotocin

diabetes rats. Journal of Nutrition Science Vitaminol 1999; 45:, 337-/346.

Choi JH, Cha BK, Rhee SJ. Effect of green tea catechin on hepatic microsomalphospholipase A2 activities and changes

of hepatic phospholipid species in streptozotocin-induced diabeticrats. Journal of Nutrinitiol Science and Vitaminol

;44: 673-/683.

Wiseman S A, Balentine D A, Frei B. Antioxidants in tea. Crit. Rev. Food Sci Nutr.1997; 37: 705-718.

Huang HF, Pogach LM, Nathan E,Giglio W. Acute and chronic effect of cisplatinum upon testicular function in the

rats. J Androl 1990; 11(5):436-45.

Gubdjorson S,Halligrimson J,Skuldottir G.Properties of transport adenosine Triphosphatase. In: Peter H.,Gresham G A.,

Paoetti R., Arterial pollution. 1983 NewYork, Plenum Publishing Corp., pp.101.

Downloads

Published

2004-12-20

How to Cite

1.
LEENA P, BALARAMAN R. Effect of green tea extract on cisplatin induced oxidative damage on kidney and testes of rats. Ars Pharm [Internet]. 2004 Dec. 20 [cited 2024 May 21];46(1):5-18. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5060

Issue

Vol. 46 No. 1 (2005)

Section

Original Articles

License

The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:

  1. The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.

b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.

c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).