Free Radical Scavenging activity of Inula cappa

Authors

  • J KALOLA Department of Phytochemistry and Pharmacognosy, L. M. College of Pharmacy, Navrangpura, Ahmedabad
  • M SHAH Department of Phytochemistry and Pharmacognosy, L. M. College of Pharmacy, Navrangpura, Ahmedabad

Keywords:

Inula cappa, Antioxidant, Chlorogenic acid, HPTLC

Abstract

Antioxidant activity (AA) of the crude extract and fractions derived from Inula cappa, was evaluated using the α, α- diphenyl- ß-picrylhydrazyl (DPPH) radical scavenging assay, superoxide radical scavenging assay, measurement ofreducing power and the measurement of effect on lipid peroxidation in rat liver homogenate, and compared with thatof butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). The results showed that the methanolicextract and the ethyl acetate-soluble fraction exhibited higher antioxidant activity. The total phenolic, total tannin andtotal fl avonoids content were also determined. Chlorogenic acid is reported for the fi rst time from this plant and a TLCdensitometric method was developed for the quantifi cation of Chlorogenic acid.

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References

Manandhar NP, An inventory of some vegetable drug resources of Makawanpur district Nepal. Fitoterapia. 1995; 66: 231-238.

Saxena VK, Saroj J, Archana S, Antibacterial activity of essential oil from the flowers of Inula Cappa. Indian Drugs. 1981, 18, 33- 35.

Jain S, Saxena VK, Antibacterial activity of the essential oil from the leaves of Inula cappa. Indian Drugs. 1983; 20: 464 -466.

Taylor RSL, Hudson JB, Manandhar NP, Towers GHN, Antiviral activities of medicinal plants of southern Nepal. J. Ethnopharmacol. 1996; 53: 97-104.

Baruah NC, Sharma RP, Thyagarajan G, Herz W, Govindan SV, New Flavonoids from Inula cappa. Phytochemistry. 1979; 18: 2003-2006.

Bohlmann F, Ahmed M, Jakupovic J, Inositol angelates from Inula cappa. Phytochemistry. 1982; 21: 780-782.

Halliwell B, How to characterize an antioxidant: an update. In: Rice-Evans, C., Halliwell B, Lunt GG, (Eds.), Free Radical and Oxidative Stress: Environments, Drugs and Food Additives. Portland Press, 1995; pp. 73–102.

Breimer LH, Molecular mechanism of oxygen radical carcinogenesis and mutagensis: the role of DNA base damage. Mol. Carcinog. 1990; 3: 188–197.

Bandoniene D, Murkovic M, On-line HPLC–DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). J. Agric. Food. Chem. 2002; 50: 2482–2487.

Fukumoto LR, Mazza G, Assessing antioxidant and prooxidant activities of phenolic compounds. J. Agric. Food Chem. 2000; 48: 3597–3604.

Singleton VL, Rossi Jr JA, Colorimetry of total phenolics with phosphomolybdic acid-phosphotungstic acid reagents. Am. J. Enol. Viticult. 1965; 16: 44–158.

William H, In: Official methods of analysis. Association of Official Agriculture Chemists, Washington DC, 1960; pp. 185.

Woisky RG, Salatino A, Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apiculture Research, 1998; 37: 99–105.

Navarro MC, Montilla MP, Martin A, Jimenez J, Utrilla MP, Free radical scavenger and antihepatotoxic activity of Rosamarinus tomentus. Planta Med. 1993; 59: 312-314.

Beauchamp C, Fridovich I, Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 1971; 44: 276–287.

Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A., Dubey GP, Bacopa monniera Linn. as an Antioxidant: Mechanism of action. Indian J. Exp. Biol. 1996; 34: 523-526.

Wagner H, Bladt S, In: Plant Drug Analysis- A Thin Layer Chromatography Atlas, Springer Verlag Berlin Heidelberg, 1996; pp. 210.

Dahl MK, Richardson T,. Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids. J. Dairy Sci. 1978; 61: 400–407.

Meyer AS, Isaksen A, Application of enzymes as food antioxidants. Trends Food Sci. Tech. 1995; 6: 300–304.

Kappus H, Lipid peroxidation—Mechanism and biological relevance. In: O. I. Aruoma, & B. Halliwell (Eds.), Free radicals and Food Additives. London: Taylor and Francis 1991.

Janero D, Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic. Biol. Med. 1990; 9: 515–540.

Published

2006-12-20

How to Cite

1.
KALOLA J, SHAH M. Free Radical Scavenging activity of Inula cappa. Ars Pharm [Internet]. 2006 Dec. 20 [cited 2024 Aug. 24];47(4):385-401. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5040

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Original Articles