HPLC method for the determination of sumatriptan in plasma and brain tissue

Authors

  • RJ MAJITHIYA New Drug Delivery Systems Laboratory, Pharmacy Department, Donor’s Plaza, Opp. to University Main Offi ce, M.S. University of Baroda, Fatehgunj, Baroda-390002, Gujarat, India
  • JB MAJITHIYA New Drug Delivery Systems Laboratory, Pharmacy Department, Donor’s Plaza, Opp. to University Main Offi ce, M.S. University of Baroda, Fatehgunj, Baroda-390002, Gujarat, India
  • ML UMRETHIA New Drug Delivery Systems Laboratory, Pharmacy Department, Donor’s Plaza, Opp. to University Main Offi ce, M.S. University of Baroda, Fatehgunj, Baroda-390002, Gujarat, India
  • PK GHOSH New Drug Delivery Systems Laboratory, Pharmacy Department, Donor’s Plaza, Opp. to University Main Offi ce, M.S. University of Baroda, Fatehgunj, Baroda-390002, Gujarat, India
  • RSR MURTHY New Drug Delivery Systems Laboratory, Pharmacy Department, Donor’s Plaza, Opp. to University Main Offi ce, M.S. University of Baroda, Fatehgunj, Baroda-390002, Gujarat, India

Keywords:

HPLC, Rat brain, Rat plasma, Sumatriptan, UV detector

Abstract

A rapid and sensitive ultra-violet high-performance liquid chromatographic method was developed and validated for thedetermination of sumatriptan, a serotonin agonist in rat plasma and brain homogenate samples. After a liquid–liquidextraction of sumatriptan and ofl oxacin (internal standard) , the compounds were separated on a reversed-phase C-18column, eluted with 22% of acetonitrile and 78% of ammonium phosphate buffer (0.04 M, adjusted pH 3.7) and detectedat 228 nm. This method shows a good linearity for plasma and brain samples with very wide concentration range of3–2000 ng/ml and 3-1000 ng/g respectively, and correlation coeffi cient of 0.9998 and 0.9994. A good precision (interand intra day CV < 9.1%) and a good accuracy (inter and intra day bias < 7.3%) was obtained with this method.The limit of quantifi cation concentration was 3 ng/ml. The absolute recovery of sumatriptan was higher than 93.4%for plasma and 89.5% for brain samples. This method is simple, rapid and sensitive. The proposed method could beadvantageous in estimation of sumatriptan incorporated into delivery systems that concentrate drug in plasma as wellas brain tissues and could be used for pharmacokinetics studies in animal model.

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Published

2006-06-20

How to Cite

1.
MAJITHIYA R, MAJITHIYA J, UMRETHIA M, GHOSH P, MURTHY R. HPLC method for the determination of sumatriptan in plasma and brain tissue. Ars Pharm [Internet]. 2006 Jun. 20 [cited 2025 Apr. 2];47(2):199-210. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/5024

Issue

Vol. 47 No. 2 (2006)

Section

Original Articles

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