Antioxidant and antibacterial activity of the extracts of Piper cubeba (Piperaceae)

Authors

  • R CHITNIS School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Co.
  • M ABICHANDANI School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Co.
  • P NIGAM School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Co.
  • L NAHAR School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Co.
  • SD SARKER School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Co.

Keywords:

Piper cubeba, Piperaceae, South-Esat Asia, Antioxidant, Antibacterial

Abstract

Piper cubeba L. (Piperaceae), commonly known as ‘cubeb’, is native to Indonesia, and also found in many other countriesof the South-East Asia, and in some African countries. This plant has been used as a popular food additive, andin folklore medicine to treat various ailments, particularly bacterial infections. The n-hexane, dichloromethane (DCM)and methanol (MeOH) extracts of the dried berries (fruit) of this plant were assessed for their antioxidant (free radicalscavenging) and antibacterial activities using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and the micro titre basedantimicrobial assay incorporating resazurin as an indicator of cell growth, respectively. While all extracts showed antioxidantactivity in the qualitative assay, the most prominent antioxidant activity was observed with the MeOH extract inthe quantitative assay with a RC50 value of 2.71 x 10-1 mg/mL. The antioxidant potency of the DCM extract was about3 fold less (RC50 = 6.50 x 10-1 mg/mL) than that of the MeOH extract. None of the extracts showed any antibacterialproperty against Bacillus subtilis, Escherichia coli, and ampicillin resistant Escherichia coli. While both the n-hexaneand the DCM extracts inhibited the growth of Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus, theMeOH extract was active only against B. cereus and P. aeruginosa. The most potent antibacterial activity was displayedby the n-hexane extract against B. cereus with an MIC value of 1.56 mg/mL. All antibacterial activities of the extractswere found to be bacteriostatic rather than bactericidal.

Downloads

Download data is not yet available.

References

GRIN Taxonomy Database. USDA, ARS, Beltsville Agricultural Research Center, Beltsville, Maryland, USA. 2007.

Available on-line at: http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?28578

Dr Duke’s Phytochemical and Ethnobotanical Databases. USDA, ARS, Beltsville Agricultural Research Center, Beltsville, Maryland, USA. 2007. Available on-line at: http://www.ars-grin.gov/cgi-bin/duke/ethnobot.pl?ethnobot. taxon=Piper%20cubeba

Elfahmi RK, Batterman S, Bos R, Kayser O, Woerdenbag HJ, Quax WJ. Lignan profile of Piper cubeba, an Indonesian medicinal plant. Biochem. Syst. Ecol. 2007: 35: 397-402.

Bodiwala HS, Singh G, Singh R, Dey CS, Sharma SS, Bhutani KK, Singh IP. Antileishmanial amides and lignans from Piper cubeba and Piper retrofractum. J. Nat. Med. 2007; 61: 418-421.

Junqueira APF, Perazzo FF, Souza GHB, Maistro EL. Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system. Genetics and Mol. Biol. 2007; 30:656-663.

Usia T, Iwata H, Hiratsula A, Watabe T, Kadota S, Tezuka Y. CYP3A4 and CYP2D6 inhibitory activities of Indonesian medicinal plants. Phytomedicine. 2006: 13: 67-73.

Matsuda H, Hirata N, Kawaguchi Y, Naruti S, Takata T, Oyama M, Inuma M, Kubo M. Melanogenesis stimulation in murine B16 melanoma cells by Kava (Piper methysticum) rhizome extract and kavalactones. Biol. Pharm. Bull. 2006; 29: 834-837.

Choi EM, Hwang JK. Investigations of anti-inflammatory and antinociceptive activities of Piper cubeba, Physalis angulata and Rosa hybrida. J. Ethnopharmacol. 2003; 89: 171-175.

Hissein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K. Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Phytotherapy Res. 2000; 14: 510-516.

Uddin SJ, Shilpi JA, Alam SMS, Alamgir M, Rahman MT, Sarker SD. Antidiarrhoeal activity of the methanol extract of the barks of Xylocarpus molucensis in castor oil and magnesium sulphate-induced diarrhoea models in mice. J. Ethnopharmacology. 2005; 101: 139-143.

Shilpi SJ, Uddin SJ, Rouf R, Billah MM. Central nervous system depressant activity of Diospyros peregrina bark. Oriental Pharmacy and Experimental Medicine. 2004; 4:249-252.

Uddin SJ, Shilpi JA, Barua J, Rouf R. Antinociceptive activity of Ceriops decandra leaf and pneumatophore. Fitoterapia. 2005; 76:261-263.

Uddin SJ, Shilpi JA, Delazar A, Nahar L, Sarker SD. Free radical scavenging activity of some Bangladeshi plant extracts. Oriental Pharmacy and Experimental Medicine. 2004; 4:185-193.

Rouf R, Uddin SJ, Shilpi JA, Rahman MT, Ferdous MM, Sarker SD. Anti-diarrhoeal effects of Diospyros peregrina in the castor oil-induced diarrhoea model in mice. ARS Pharmaceutica. 2006; 47: 81-89.

Uddin SJ, Shilpi JA, Rahman MT, Ferdous MM, Rouf R, Sarker SD. Assessment of neuropharmacological effects of Pandanus foetidus (Pandanaceae) in mice. Die Pharmazie. 2006; 61: 262-264.

Datta BK, Nahar L, Rahman MM, Gray AI, Auzi AA, Sarker SD. Polygosumic acid, a new cadinane sesquiterpene, from Polygonum viscosum inhibits the growth of drug-resistant Escherichia coli and Staphylococcus aureus (MRSA) in vitro. J. Nat. Med. 2007; 61:391-396.

Saha A, Masud MA, Bachar SC, Kundu JK, Nahar L, Datta BK, Sarker SD. Analgesic and anti-inflammatory activities of the extracts of Phyllanthus reticulatus in mice model. Pharm. Biol. 2007; 45: 355-359.

Uddin SJ, Shilpi JA, Rouf R, Ferdous MM, Nahar L, Sarker SD. Neuropharmacological properties of Xylocarpus molucensis. Fitoterapia 2007; 78: 107-111.

Uddin SJ, Shilpi JA, Byres M, Middleton M, Shoeb M, Nahar L, Sarker SD. Swarnalin and cis-swarnalin, two new tetrahydrofuran derivatives with free radical scavenging activity, from the aerial parts of Cuscuta refl exa. Nat. Prod. Res. 2007; 21: 663-668.

Mondal S, Paul SK, Uddin SJ, Nahar L, Auzi AA, Sarker SD. A comparative study on the in vitro antibacterial activity of the pneumatophores of Heritiera fomes and Xylocarpus moluccensis. ARS Pharmaceutica. 2007; (in press).

Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for freeradical scavenging activity. Phytotherapy Res. 2007; 21: 615-621.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007; 42: 321-324.

Takao T, Watanabe N, Yagi I, Sakata K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci. Biotech.Biochem. 1994; 58: 1780-1783.

Bauer AW, Kirby WMM, Sherris JC, Truck M. Antimicrobial susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966; 45: 493-496.

Cruickshank R. Medical microbiology: A guide to diagnosis and control of infection. E. and S. Livingstone Ltd., Edinburgh and London, 1968; p.888.

Choi EM, Hwang JK. Effect of some medicinal plants on plsama antioxidant system and lipid levels in rats. Phytotherapy Res. 2005; 19: 382-386.

Silva MLA, Coimbra HS, Pereira AC, Almeida VA, Lima TC, Costa ES, Vinholis AHC, Royo VA, Silva R, Filho AAS, Cunha WR, Furtado NAJC, Maryins CHG, Carvalho TC, Bastos JK. Evaluation of Piper cubeba extract, (-)-cubebin and its seni-synthetic derivatives against oral pathogens. Phytotherapy Research. 2007; 21: 420-422.

Downloads

Published

2007-12-20

How to Cite

1.
CHITNIS R, ABICHANDANI M, NIGAM P, NAHAR L, SARKER S. Antioxidant and antibacterial activity of the extracts of Piper cubeba (Piperaceae). Ars Pharm [Internet]. 2007 Dec. 20 [cited 2024 Jul. 22];48(4):343-50. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4995

Issue

Vol. 48 No. 4 (2007)

Section

Original Articles

License

The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:

  1. The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.

b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.

c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).