Staphylococcal enterotoxins and enzymes in presence of essential oils

Authors

  • L NUÑEZ Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, (1153) Buenos Aires, Argentina.
  • A MORO Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, (1153) Buenos Aires, Argentina.
  • M D’AQUINO Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, (1153) Buenos Aires, Argentina.

Keywords:

Clove, Cinnamon, Thyme, M Staphylococcus aureus, Enzymatic activity, Enterotoxin

Abstract

Our aim was to investigate the effect of subinhibitory concentrations of clove, cinnamon, and thyme essential oils onthe growth of Staphylococcus aureus and its production of coagulase, thermonuclease and enterotoxin. Different concentrationsof the essential oils were added to Brain Heart Infusion broth. The production of enterotoxin in the presenceor absence of essential oils was estimated using the ELISA technique. Our results indicate that a lower concentrationdid not affect the growth S. aureus but reduced the production of metabolites. Thyme oil presents the most signifi canteffect, inhibiting the activity of both enzymes and the production of enterotoxin with 0,04%. There is a renewed interestin the potential application of these essential oils in the food and pharmaceutical industries to control staphylococcalpoisoning.

Downloads

Download data is not yet available.

References

Arora DS, Kaur J. Antimicrobial activity of spices. Int J Antimicrob Agents 1999; 12: 237-262.

Chao SC, Young DG. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research 2000;12: 639-649.

Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000; 88: 308-316.

Tranter HS, Tassou SC, Nychas GJ. The effect of the olive phenolic compoud, oleuropein, on growth and enterotoxin B production by Staphylococcus aureus. J. Appl. Bacteriol.1993, 74: 253-259.

González-Fandos E, García- López ML, Sierra ML, Otero O. Staphylococcal growth and enterotoxins (A-D) and thermonucleasa

syntesis in the presence of dehydrated garlic. J. Appl. Bacteriol.1994; 77: 49-552.

Nostro A, Bisignano G, Cannatelli MA, Crisafi G, Germanó MP, Alonzo V. Effect of Helichrysum italicum extract on growth and enzimatic activity of Staphylococcus aureus. Int. J. Antimicrob. Agents 2001; 17: 517-520.

Nostro A, Cannatelli MA, Musolino AD, Procopio F, Alonzo V. Helichrysum italicum extract interferes with the production of enterotoxins by Staphylococcus aureus. Lett. Appl. Microbiol. 2002; 35: 181-184.

Smith-Palmer A, Stewart J, Fyfe L. Inhibition of listeriolysin O and phosphatidylcholine- specific production in Listeria monocytogenes by subinhibitory concentrations of plant essential oils. J. Med. Microbiol. 2002; 51: 567- 574.

Novick RP. Pathogenicity factors and their regulation. In Gram-Positive Pathogens, Fischetti, VA. et al (ed.), pp.392-406. American Society for Microbiology, Washington, D.C.2000

Dinges MM, Orwin PM, Schlievert P. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 2000; 13: 16-34.

Jablonski LM, Bohach GA. Staphylococcus aureus. In Food Microbiology. Fundamentals and Frontiers. ed. Doyle M, Beuchat LR, Montville TJ. ASM Press. Washington D.C.1997.

Sperber WH, Tatini SR. Interpretation of the coagulase test for identification of Staphylococcus aureus. Appl.Microbiol. 1975; 29: 502-505.

Lachica RVF, Genigeorgis C, Hoeprich. Metachromatic Agar - diffusion methods for detecting Staphylococcal Nuclease activity. Appl. Microbiol. 1971; 21: 585-587.

Hili P, Evans CS, Veness RG. Antimicrobial action of essential oils: the effect of dimethylsulphoxide on the activity of cinnamon oil Lett. Appl. Microbiol. 1997; 24: 269-275.

Sikkema J, De Bont J, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews. 1995; 59: 201-222.

Lambert RJW, Skandamis PN, Coote PJ, Nychas JE. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol. 2001; 91: 453-462.

Fishman Y, Rottem S, Citri N. Preferencial suppression of normal exoenzyme formation by membrane- modifying agents. J. Bacteriol. 1980;114: 1435-1438.

Published

2007-06-16

How to Cite

1.
NUÑEZ L, MORO A, D’AQUINO M. Staphylococcal enterotoxins and enzymes in presence of essential oils. Ars Pharm [Internet]. 2007 Jun. 16 [cited 2024 Jul. 22];48(2):175-8. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4984

Issue

Section

Original Articles