High-performance liquid chromatography method for the quantifi cation of duloxetine in rat plasma

Authors

  • S LAKSHMANA PRABU Manipal College of Pharmaceutical Sciences, Manipal – 576 104. Karnataka, India
  • S SHAHNAWAZ Manipal College of Pharmaceutical Sciences, Manipal – 576 104. Karnataka, India
  • A KARTHIK Manipal College of Pharmaceutical Sciences, Manipal – 576 104. Karnataka, India
  • C DINESH KUMAR Manipal College of Pharmaceutical Sciences, Manipal – 576 104. Karnataka, India
  • SG VASANTHARAJU Manipal College of Pharmaceutical Sciences, Manipal – 576 104. Karnataka, India

Keywords:

Duloxetine, Trifluoperazine, HPLC, Validation, Rat Plasma

Abstract

A sensitive and selective HPLC method was developed for quantifi cation of duloxetine, in rat plasma. Trifl uoperazinewas used as an internal standard (IS). The present method used protein precipitation for extraction of the drug fromrat plasma. Separation and quantifi cation was carried using in isocratic mode using 25 mM phosphate buffer (pH3.0)/acetonitrile (60:40, % v/v) as mobile phase and on reverse-phase C18 phenyl column (250 mm x 4.6 mm, 5μ) andthe column effl uent was monitored by UV detector at 217 nm. This method was linear over the range of 44 - 2816.00ng/ml with regression coeffi cient greater than 0.99. The mean recovery of duloxetine and IS were 82.33 ± 2.10 and75.37 ± 1.07, respectively and the method was found to be precise, accurate and specifi c during the study. This validatedmethod is sensitive and reproducible and it can be used for pharmacokinetic studies.

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Published

2008-12-20

How to Cite

1.
LAKSHMANA PRABU S, SHAHNAWAZ S, KARTHIK A, DINESH KUMAR C, VASANTHARAJU S. High-performance liquid chromatography method for the quantifi cation of duloxetine in rat plasma. Ars Pharm [Internet]. 2008 Dec. 20 [cited 2024 Jul. 22];49(4):283-92. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4973

Issue

Vol. 49 No. 4 (2008)

Section

Review Articles

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