Design of shark skin collagen-aloe composite scaffold for tissue engineering

Authors

  • S SHANMUGANATHAN Biomaterials Division, Central Leather Research Institute, TICEL Biopark, Taramani Road, Taramani, Chennai – 600 113, Tamil Nadu, India. Department of Pharmaceutics, Sankaralingam Bhuvaneswari College of Pharmacy, Sivakasi - 626130, Tamil Nadu, India.
  • N SHANMUGASUNDARAM US Army Institute of Surgical Research, Texas, USA
  • N ADHIRAJAN Biomaterials Division, Central Leather Research Institute, TICEL Biopark, Taramani Road, Taramani, Chennai – 600 113, Tamil Nadu, India.
  • TS RAMYAA LAKSHMI Biomaterials Division, Central Leather Research Institute, TICEL Biopark, Taramani Road, Taramani, Chennai – 600 113, Tamil Nadu, India.
  • M BABU Biomaterials Division, Central Leather Research Institute, TICEL Biopark, Taramani Road, Taramani, Chennai – 600 113, Tamil Nadu, India.

Keywords:

Collagen, Scaffold, Biocompatibility, Glycosaminoglycan, Aloe vera

Abstract

Collagen has proven to be a novel biomaterial used for drug delivery, wound cover dressings or as a substrate for tissueengineering with unique biocompatibility and biodegradable properties. Bovine and porcine Type I collagen providea readily available source of scaffold material for various biomedical applications. However these sources have somepotential risk of infectious diseases such as bovine spongiform encephalopathy or transmissible spongiform encephalopathy.Hence there is demand for an alternative Type I collagen from various other sources. The present study utilizesthe aquatic animals particularly the shark species in which collagen Type I is a major protein in the skin and thestructure has similarity to that of mammalian species. An attempt was made to use shark skin collagen as scaffold withthe extract of aloe to improve the stability. These scaffolds were characterized for various physicochemical propertiesand biocompatibility assessment to support the growth of human dermal fi broblasts in vitro. The incorporation of aloe extract highly infl uenced the morphology and physicochemical properties of the scaffold. It was observed in vitro thatthe fi broblasts retained the spindle shape, organized orientation when cultured over collagen scaffold. Thus the developedcollagen scaffold at 10: 1 ratio of shark skin collagen and aloe extract respectively served as a biocompatible materialwith appreciable tensile strength. The above investigation suggests that the developed shark skin collagen scaffold could bean effective alternative for the mammalian collagen for tissue engineering and various wound healing applications.

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Published

2008-12-20

How to Cite

1.
SHANMUGANATHAN S, SHANMUGASUNDARAM N, ADHIRAJAN N, RAMYAA LAKSHMI T, BABU M. Design of shark skin collagen-aloe composite scaffold for tissue engineering. Ars Pharm [Internet]. 2008 Dec. 20 [cited 2025 Jun. 20];49(4):321-40. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4970

Issue

Vol. 49 No. 4 (2008)

Section

Original Articles

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