Radioactive immunocomplex formation in a double antibody ria

Authors

  • C Olivas Arroyo Servicio de Medicina Nuclear. Unidad de Radiofarmacia. Hospital Clínico Universitario de Valencia
  • Jl Moreno Frigols Servicio de Medicina Nuclear. Unidad de Radiofarmacia. Hospital Clínico Universitario de Valencia Departamento de Química Física. Facultad de Farmacia. Universidad de Valencia

Keywords:

Kinetics, RIA, Double Antibody, C Peptide

Abstract

A model has been produced for the kinetic and equilibrium study of antigen-antibody reactions in the radioimmunoassay (RIA) of C-Peptide double antibody. The aim is (1) to characterise the influence of initial concentrations of labelled (M) and unlabelled (Q) antigen and that of the initial concentration of the antibody in solution (P), and (2) to study the binding of the immunocomplex (PM) to the second antibody (J) immobilised on a bead, and the replacement of M by Q in the PMJ immunocomplex. In order to study the effect of such variables, 44 experiments were conducted. The results are in line with the model proposed.

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Published

2009-03-20

How to Cite

1.
Olivas Arroyo C, Moreno Frigols J. Radioactive immunocomplex formation in a double antibody ria. Ars Pharm [Internet]. 2009 Mar. 20 [cited 2024 May 17];50(4):235-47. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4909

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Original Articles