Hook effect in radioligand assay for Anti Glutamic Acid Decarboxylase (Anti-GAD65). Influence of temperature and physicochemical interpretation

Authors

  • T Peris-Conejero Nuclear Medicine Service. Radiopharmacy Unit. Hospital Clínico Universitario, Valencia, Spain
  • R Olivares-Pallerols Nuclear Medicine Service. Radiopharmacy Unit. Hospital Clínico Universitario, Valencia, Spain
  • H García-Ruiz Nuclear Medicine Service. Radiopharmacy Unit. Hospital Clínico Universitario, Valencia, Spain
  • Jl Moreno-Frigols Nuclear Medicine Service. Radiopharmacy Unit. Hospital Clínico Universitario, Valencia, Spain Department of Physical Chemistry, Faculty of Pharmacy. University of Valencia, Spain

Keywords:

Calibration curves, Anti-GAD65, Hook effect

Abstract

Background: Radioligand assay is one of the principal methods used for analyticaldetermination of the Anti-GAD65 concentration. We studied the influence of temperature onthe calibration curves obtained by such a method

Matherial and Methods: We used a commercially available RIA kit for Anti-GAD65 and agamma counter. Data was analyzed using Statistica software.

Results and Discusion: Activities bound to the antibody increase with temperature. Therewas a decrease in activity for high concentrations attributable to the "hook effect". Wepropose a simple physicochemical model that justifies satisfactorily the results

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References

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Published

2009-06-20

How to Cite

1.
Peris-Conejero T, Olivares-Pallerols R, García-Ruiz H, Moreno-Frigols J. Hook effect in radioligand assay for Anti Glutamic Acid Decarboxylase (Anti-GAD65). Influence of temperature and physicochemical interpretation. Ars Pharm [Internet]. 2009 Jun. 20 [cited 2024 May 12];50(2):76-84. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4887

Issue

Vol. 50 No. 2 (2009)

Section

Original Articles

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