Comparison of the Sprague Dawley rat’s response against cyclophosphamide and bleomycin in the head sperm morphology assay.

Authors

  • A Vidal Novoa Facultad de Biología (U.H)
  • LA Rosario Fernández Departamento de Microbiología, Instituto de Farmacia y Alimentos (IFAL, U.H)
  • L Delgado Roche Centro de Estudios para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos, Universidad de La Habana

Keywords:

Bleomycin, cyclophosphamide, head sperm morphology assay induction, Sprague Dawley rat,

Abstract

The head sperm morphology assay, allows the study of the genotoxic potencial of samples at thecellular level problems; this tool to evaluate the spermatic concentration changes, as well as theincrease of the spontaneous heads frequency of sperms morphology abnormal. In this article we decideto evaluate and to compare in the Sprague Dawley males rats, the effect of two mutagenic substancesin these assay, the Cyclophosphamide, (CF) in dose of 50 mg/kg and the Bleomycin (BL) in dose of30 mg/kg, administered by intraperitoneal route during 5 consecutive days, being sacrificed theanimals 52 days after the last administration of the mutagens. It was obtained that both mutagendiminished the spermatic concentration and they increased the spontaneous index of anomaloussperms heads, being observed a marked mutagenic effect in the CF in comparison with the BL. At theend of the experience bigger results of induction of anomalous heads sperms were obtained with theuse of the CF. These results reaffirm the use of this mutagen as efficient and safe positive control inthis assay which is included in the genotoxicity studies and fertility toxicology.

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Published

2010-09-20

How to Cite

1.
Vidal Novoa A, Rosario Fernández L, Delgado Roche L. Comparison of the Sprague Dawley rat’s response against cyclophosphamide and bleomycin in the head sperm morphology assay. Ars Pharm [Internet]. 2010 Sep. 20 [cited 2024 Jul. 22];51(3):155-62. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4871

Issue

Vol. 51 No. 3 (2010)

Section

Original Articles

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