A comparison of methods for the quality control of 123I-Ioflupane

Authors

  • MH Ezz_Eddin Unidad de Radiofarmacia. Servicio de Medicina Nuclear. Hospital Clínico Universitario de Valencia.
  • L Díaz Platas Unidad de Radiofarmacia. Servicio de Medicina Nuclear. Hospital Clínico Universitario de Valencia.
  • JL Moreno Frigols Unidad de Radiofarmacia. Servicio de Medicina Nuclear. Hospital Clínico Universitario de Valencia. Departamento de Química Física. Facultad de Farmacia. Universidad de Valencia.

Keywords:

Radiocromatography, Thin layer, Rf, Radiochemical purity, Ioflupane, Control quality

Abstract

Aim: The aim of this study is to stablish an easy, reproducible, and precise method to determine the radiochemical purity of 123I-Ioflupane.

Material and methods: Different systems of radiochromatography were used with three types of stationary phases (Whatmann-17MM, Whatmann-3MM and ITLC-SG) and five mobile phases (Diethylether, NaCl 0.9%, Methanol:Chloroform(1:9), Methanol:Water (85:15) and Methylethylketone). This procedure was made with samples of 123I-Ioflupane and 123I-NaI to study the behaviour of free iodide.

Results: Whatmann-3MM was rejected due to an unsuccessful separation and Methanol:Water (85:15) with ITLC-SG was not reproducible. Free fraction wasn`t observed in Methylethylketone as mobile phase with ITLC-SG and Whatmann-17MM. Free fraction was just observed in Whatmann-17MM with Methanol:Cloroform and Diethylether and in ITLC-SG with NaCl 0.9%.

Conclusions: Maximum separation between 123I-Ioflupane and 123I- was observed in Whatmann-17MM/Methanol:Cloroform(1:9) (Rf of bound fraction = 0.62±0.03, Radiochemical Purity =91.6±2.36%), ITLC-SG/NaCl 0,9% (Rf of bound fraction = 0.21±0.05, Radiochemical Purity =90.19±3.4%) and Whatmann-17MM/ Diethylether (Rf of bound fraction = 0.62±0.06, Radiochemical Purity =92.79±1.65%).

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References

Martins P, Silva J, Ramos M, Oliveira I, Felgueiras C, Herrerias

R et al. Radiochemical stability of radiopharmaceutical preparations. En: Associaçao Brasileira de Energía Nuclear. Ponencias de 2011 International Nuclear Atlantic Conference; 24-28 octubre 2011; Belo Horizonte, Brazil; 2011.

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Rankin A, Zimmer M, Riehle L, Spies S.Rapid miniaturized

chromatography system for I-123 Ioflupane. J Nucl Med. 2012; 53 Supl 1: 2729.

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Published

2013-09-20

How to Cite

1.
Ezz_Eddin M, Díaz Platas L, Moreno Frigols J. A comparison of methods for the quality control of 123I-Ioflupane. Ars Pharm [Internet]. 2013 Sep. 20 [cited 2024 Jul. 22];54(3):52-5. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4648

Issue

Vol. 54 No. 3 (2013)

Section

Short Articles

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