Phyllanthus orbicularis K aqueous extract protect plasmid DNA against the damage induced by ultraviolet radiation.

Authors

  • M Vernhes Departamento de Radiobiología, (CEADEN) (Habana, Cuba.)
  • M González-Pumariega Laboratorio de Genética Toxicológica, Departamento de Biología Vegetal, Universidad de la Habana, (Habana, Cuba)
  • A Passaglia Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, (São Paulo, SP, Brazil)
  • FC Martins Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, (São Paulo, SP, Brazil)
  • A Sánchez-Lamar Laboratorio de Genética Toxicológica, Departamento de Biología Vegetal, Universidad de la Habana, (Habana, Cuba)

Keywords:

Phyllanthus Orbicularis, UV, pyrimidine dimers, vegetal extract, photoprotection

Abstract

Aim: The aim of this work was to evaluate the protective effect of aqueous extract of Phyllanthus Orbicularis, K from the damage induced by UV radiation.

Material and methods: The plasmid-based non cellular system was used. The extract capacity to induce DNA strand breaks was evaluated at 0.0001- 2.0 mg/ml concentrations. The protective effect of extract against UVB and UVC radiation was evaluated at 0.1- 2.0 mg/ml concentrations. The transmittance of extract was measured for both UV radiations.

Results: The P. orbicularis aqueous extract was not genotoxic even after 30 min of exposure. Concentrations ≥ 1 mg/ml of extract induced strand breaks at major times of exposition. P. Orbicularis extract protected DNA against UVB and UVC radiation at concentrations ≥ 0.1 mg/ml and 0.5 mg/ml respectively.

Conclusions: In our experimental conditions, P. Orbicularis aqueous extract protects DNA from damage induced by UV radiation.

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Published

2013-03-20

How to Cite

1.
Vernhes M, González-Pumariega M, Passaglia A, Martins F, Sánchez-Lamar A. Phyllanthus orbicularis K aqueous extract protect plasmid DNA against the damage induced by ultraviolet radiation. Ars Pharm [Internet]. 2013 Mar. 20 [cited 2024 Aug. 24];54(1):16-23. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/4577

Issue

Vol. 54 No. 1 (2013)

Section

Original Articles

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