Comparation of antioxidants properties and polyphenols content of aqueous extract from seaweeds Bryothamnion triquetrum and Halimeda opuntia
Keywords:
seaweed, antioxidants, Halimeda opuntia, Bryothamnion triquetrum, polyphenolAbstract
Objective. To evaluate and compare the antioxidant activity displayed by seaweed H. opuntia and B.triquetrum using different experimental in vitro antioxidant assessment models.
Material and Methods.The following techniques are utilized: DPPH, Reducing power, inhibition oflipid peroxidation and inhibition of haemolysis.
Results. B. triquetrum: DPPH; IC50=1.15 ± 0.06, Reducing power (concentration 128 mg/mL), OD=2.798,inhibition of lipid peroxidation; IC50=5.09± 0.25 and inhibition of haemolysis; with 12 mg/mL; 35 %. H.opuntia: DPPH; IC50=12.34 ± 0.30 mg/mL, reducing power; OD=0.800, inhibition of lipid peroxidation;IC50=1.25± 0.31 mg/mL, inhibition of lipid peroxidation; IC50=1.25± 0.31 mg/mL and inhibition of haemolysis;82%.
Conclusion. It was demonstrated that B.triquetrum extract was more effective than H. opuntia in reducingpower and DPPH assays while H.opuntia was more effective in inhibition of lipid peroxidation in ratbrain homogenates and the inhibition of red blood cell (RBC) haemolysis induced by AAPH. We discusssome aspects about their possible mechanisms of action.
Downloads
References
Mac Artain P, Gill CIR, Brooks M, Campbell R, Rowland IR. Nutritional value of edible seaweeds. Nutr Rev. 2007; 65(12): 535-43.
Roig JT. Plantas medicinales, aromáticas o venenosas de Cuba. 1ª ed. Cuba: Editorial Ciencia y técnica, La Habana; 1974.
Proksch P, Edrada-Ebel RA, Ebel R. Drugs from the sea- Opportunities and obstacles. Mar Drugs. 2003; 1: 5-17.
Gomez –Gutierrez CM, Guerra-Rivas G, Soria-Mercado IE, Ayala-Sánchez NE. Marine edible algae as disease preventers. Adv Food Nutr Res. 2011; 64: 29-39.
Sampth-Wiley P, Neefus CD, Jahnke LS. Seasonal effects of sun exposure and emersion on intertidal seaweed physiology: fluctuations in antioxidant contents, photosynthetic pigments and photosynthetic efficiency in the red alga PhorphyraumbilicalisKutzing (Rodophyta, Bangiales). J Exp Mar Biol Ecol. 2008; 36: 83-91.
Dutra Rocha F, Crespo Pereira R, Coelho Kaplan MA, Laneuville Teixeira V. Produtos naturais de algas marinhas e sue potencial antioxidante. Braz J Pharmacogn. 2007;17(4): 631-39.
Fallarero A, Peltoketo A, Loikkanen JJ, Tammela P, Vidal A, Vuorela P. Effects of aqueous extracts of Bryothamnion triquetrum on chemical hypoxia and aglycemia-induced damage in GT1-7 mouse hypothalamic immortalized cells. Phytomedicine 2006; 13(4):240-45.
Rivero F, Fallararo A, Castañeda O, Dajas F, Manta E, Areces A, et al. Antioxidant activity in vivo and in vitro of Halimeda incrassata aqueous extracts. Cienc Tecnol Alimentos (Campinas) 2003; 23: 256 –63.
Vidal A, Silva de Andrade-Wartha ER, de Oliveira e Silva AM, Pavan R, Lima A, Fallarero A, et al. Actividad antioxidante y polifenoles de algas marinas verdes Halimedaopuntiay Halimeda monile. Ars Pharm. 2009; 50 (1): 24-31.
Batista-Gonzalez AE, de Oliveira e Silva AM, Vidal-Novoa A, Pinto JR, Portari Mancini DA, Mancini-Filho J. Analysis of antioxidant properties of hydrophilic fractions from seaweed Halimeda monileL. and its function in vivo. J Food Biochem. 2012; 36 :189–97.
Silva AM de O, Vidal-Novoa A, Batista-González AE, Pinto JR, Portari Mancini DA, Reina-Urquijo W, et al. Antioxidant activity and hepatoprotectivie properties of polyphenols in vitro and in vivo from seaweeds Halimeda opuntia (Linnaeus) Lamouroux. Redox report. 2012;17 (2): 47-53.
Vidal A, Motidome M, Mancini J, Fallarero A, Tanae MM, Brandao LM, et al. Actividad antioxidante y ácidos fenólicos del alga marina Bryothamnion triquetrum (SG Gmelim) Howe. Braz J Pharm Sci. 2001; 37(3), 373–82.
Oyaizu M. Studies on products of browning reaction: Antioxidant activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986; 44: 307-15.
Brand-Williams W, Cuvelier ME, Berset C. Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm.-Wiss. u.-Technol. 1995; 28:25-30.
Ohkawa H, Noburo O, Kunio Y. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-58.
Aman S, Moin S, Owanis M, Siddiqui MU. Antioxidant activity of thymol: protective role in AAPH-induced hemolysis in diabetic erythrocytes. IJPSI, 2013; 2 (3): 55-60.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982; 45:31-4.
Vauzour D, Rodriguez-Mateos A, Corona G, Oruna-Concha MJ, Spencer JPE. Polyphenols and human health: prevention of disease and mechanisms of action. Nutrients. 2010; 2: 1106-31.
Kuda T, Ikemori T. Minerals, polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beach-casts in the Noto Peninsula, Ishikawa, Japan. Food Chem. 2009; 112: 575-81.
Zubia M, Robledo D, Freile-Pelegrin Y. Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. J Appl Phycol. 2007; 18: 449–58.
Costa-Mugica A, Batista-Gonzalez AE, Mondejar D, Soto-López Y, Brito-Navarro V, Vázquez AM, et al. Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux. Braz J Pharm Sci. 2012; 48 (1): 31-7.
Yoshie Y, Wang W, Hsieh YP, Suzuki T. Compositional difference of phenolic compounds between two seaweeds, Halimeda spp. J Tokyo Univ Fish.. 2002; 88: 21-4.
Vidal A, Fallarero A, de Andrade-Wartha ERS, de Oliveira e Silva AM, de Lima A, Pavan R, et al. Composición química y actividad antioxidante del alga marina roja Bryothamnion triquetrum (S.G.Gmelin) Howe. Braz J Pharm Sci. 2006; 43(4): 589-600.
Nakai M, Kageyama N, Nakahara K, Miki W. Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum. Mar Biotechnol. 2006; 8: 409-14.
Zubia M, Fabre MS, Kerjean V, Deslandes E. Antioxidant and cytotoxic activities of some red algae (Rhodophyta) from Brittany coasts (France) Bot Mar. 2009; 52: 268-77.
Moon J-K, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009; 57(2): 1655-66.
Boonchum W, Peerapornpisal Y, Kanjanapoth D, Pekkoh J, Pumas C, Jamjai U, et al. Antioxidant activity fo some seaweed from the Gulf of Tailand. Int J Agric Biol. 2011; 13(1):95-9.
Kaur G, AlamS, Jabbar Z, Javed K, Athar M. Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol. 2006; 108:340-8.
Lim CS, Jin DQ, Sung JY, Lee JH, Choi HG, Há I, et al. Antioxidant and anti-inflamatory activities of the methanolic extract of Neorhodomela aculeate in Hippocampal and Microglial cells. Biol Pharm Bull. 2006; 29(6): 1212–6.
Matsukawa R, Dubinsky Z, Kishimoto E, Masaki K, Masuda Y, Takeuchi T, et al. A comparison of screening methods for antioxidant activity in seaweeds. J Appl Phycol. 1997; 9: 29-35.
Chakraborty K, Praveen NK, Vijayan KK, Rao GS. Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar. Asian Pac J Trop Biomed. 2013; 3(1):8-16.
Lim SN, Cheung PCK, Ooi VEC, Ang PO. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J. Agric Food Chem. 2002; 50:3862-6.
Pannangpetch P, Laupattarakasem P, Kukongviriyapan V, Kukongviriyapan U, Kongyingyoes B, Aromdee C. Antioxidant activity and protective effect against oxidative hemolysis of Clinacanthus nutans (Burm.f) Lindau. Songklanakarin. J Sci Technol. 2007; 29 (Suppl. 1): 1-9.
Benites Vílchez J, Díaz García R, López Vivar J, Gajardo Solari S, Kusch Fuschlocher F, Rojas Arredondo M. Actividad antioxidante y antibacteriana de seis cáscaras de frutos del oasis de Pica. Biofarbo. 2011; 19(1):1-7.
Puppo A. Effect of flavonoids on hydroxyl radical formation by Fenton-type reactions; influence of the iron chelator. Phytochemistry. 1992; 31: 85–8.
Anantharaman P, Karthikaidevi G, Manivannan G, Thirumaran G, Balasubramanian T. Mineral composition of marine macroalgal from Mandapam coastal regions; Southeast Coast of India. Rec Res Sci Tech. 2010; 2(10): 66-71.
Freile-Pelegrin Y, Robledo D, Chan-Bacab MJ, Ortega-Morales BO. Antileishmanial properties of tropical marine algae extracts. Fitoterapia. 2008; 79: 374-7.
Ara J, Sultana V, Ehteshamul-Haque S, Qasim R, Viqar Uddin Ahmad VU. Cytotoxic activity of marine macro-algae on Artemia salina (Brine Shrimp). Phytother Res. 1999; 13: 304–7.
Downloads
Published
How to Cite
Issue
Section
License
The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:
- The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.
b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.
c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).