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Abstract
When a radioimmunoasay method is used to analyze samples of a type different than these for wich it was developed, the method needs to be validated to asses the absence of analytical interferences even if antigenic identity exist. We have perfomed validation tests (parallelism, recovery and non specific binding) when applying two comercial RIA methods for clínical determination of thyroid hormones (T3, T4) to rat serum samples. The T4 shows lack of parallelism between the samples analyzed and the standard curve, the recovery reaches values of 147,5%, so we infer the existence of analytical interferences in the RIA assayed wich precludes obtaining valid results when used to analyze rat serum samples. The T4 RIA assayed fullfills to satisfaction the parallelism test, the average recovery obtained is 95,7% and NSB in rat serum is similar to that of the standard curve, so we conclude this T). RIA is able for the sample analysis of rat serum.
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