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Abstract
Fatty acid composition in rat red blood cell membranes and hepatic microsomes was determined, as well as the effect that a storage time at -36°C and freezing and thawing have had on the acidic composition of samples. Likewise, the influence that two different hypotonic buffer, used in obtaining red bolld cell membranes, have had on stability against oxidation of fatty
acids has been studied. Our results demonstrate a higher stability of fatty acid microsomes than of red blood cell membranes, aswell as showing that it is advisable to use a hipotonic Tris buffer instead of a Phosphate buffer when preparing red blood cell membranes.
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