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Abstract
The conservation of bacterial strains adscribed to different genus and species, by freezing at -300ºC using disc paper of cellulose have been studied. It has also tested wether this technique can be utilized for maintenance of rnicroorganisms with structural and physiologic differences. The results show how the freezing of bacterial cultures on cellulose disc paper, keep the bacterial viability in good conditions. This technique is a good way of mainteneance of bacterial strains. It is significative that for recuperation of non halophiles strains, no enrichment medium is required.
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