Lipoprotein lipase and horrnone-sensitive lipase activity and mRNA expression in tissues of virgin and pregnant diabetics rats

Authors

  • L. BLANCO-DOLADO
  • E. Herrera
  • A. MARTÍN-HIDALGO

Keywords:

gestation, lipoprotein lipase, hormone-sensitive lipase

Abstract

In order of studying the effect of gestation and diabetes on tissue lipoprotein

lipase (LPL) and hormone-sensitive lipase (HSL) activities and rnRNA expression, rats

were treated with 40 mg streptozotocin/kg and killed at day 20 of gestation. LPL

activity and rnRNA level in white adipose tissue (W AT) were lower in control pregnant

rats (CP) than in control virgin rats (CV), the activity decreasing in diabetic rats with regard to control rats (C), both pregnants (P) and virgins (V). LPL activity and mRNA

level in marnmary gland (MG) was higher in CP that CV, and it was lower in DV and

DP with respect to C. HSL activity in W AT was higher in P than in V, whereas did not

differ between CP and DP and no changes were found in mRNA levels. In MG both

mRNA level and HSL activity were lower in P than V, and it was lower in D than in

C rats. We concluded that the mechanism of regulation of LPL and HSL activities and

molecular expression during gestation and diabetes are tissues-specific. The paralellism

between LPL activity and mRNA level in WAT and MG, and between HSL activity and

mRNA level in MG indicates that transcriptional regulated-genes expression could be

an important mechanism for the tissue triglyceride uptake and mobilization in these

tissues

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References

HERRERA, E., LASUNCIÓN, M. A., GÓMEZ-CORONADO, D., ARANDA, A., LÓPEZLUNA,
P. and MAIER, 1.: "Role oflipoprotein lipase activity on lipoprotein metabolism
and the fate of circulating triglycerides in pregnancy". Am J Obstet Gyneco! (1988),
156: 1575-1583.

ARGILES, J., HERRERA, E.: "Lipids and lipoproteins in maternal and fetus plasma in
the rat". Bio! Neonate (1981), 39:37-44.

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Published

1996-12-20

How to Cite

1.
BLANCO-DOLADO L, Herrera E, MARTÍN-HIDALGO A. Lipoprotein lipase and horrnone-sensitive lipase activity and mRNA expression in tissues of virgin and pregnant diabetics rats. Ars Pharm [Internet]. 1996 Dec. 20 [cited 2024 May 19];37(4):945-56. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21761

Issue

Vol. 37 No. 4 (1996)

Section

Original Articles

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