Quinolone resistance mechanism in Salmonella typhimurium strains

Authors

  • D. CASTRO
  • J. RUIZ
  • J.A. SANTAMARÍA
  • J. VILA
  • J. J. BORREGO

Keywords:

Salmonella typhimurium., Quinolones, Resistance, PCR, ParC, GyrA

Abstract

The mechanism of quinolone resistance in 42 food-borne strains of Salmonella typhimurium which showed nalidixic acid resistance was investigated. Analysis of outer membrane proteins and Iipopolysaccharides from both quinolone-susceptible and resistant did not shown any differences among the clinical isolates tested. A 285 bp PCR fragment containing the quinolone resistance determining region of the gyrA gene and a 237 bp PCR fragment containing the region of parC homologous to the quinolone-resistance determining region of the gyrA gene, were sequenced. All the resistant strains presented a change at Ser-83 to Phe in the GyrA protein, but no changes were observed in the ParC protein. These findings indicate that this mutation in gyrA gene plays a major role in the acquisition of nalidixic acid resistance in clinical isolates of S. typhimurium.

 

Downloads

Download data is not yet available.

Downloads

Published

1997-01-20

How to Cite

1.
CASTRO D, RUIZ J, SANTAMARÍA J, VILA J, BORREGO JJ. Quinolone resistance mechanism in Salmonella typhimurium strains . Ars Pharm [Internet]. 1997 Jan. 20 [cited 2024 Jul. 22];38(1):63-9. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21407

Issue

Vol. 38 No. 1 (1997)

Section

Original Articles

License

The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:

  1. The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.

b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.

c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).