Nucleoside transport systems in liver parenchymal cells. Isoform expression pattern in hepatocyte differentiation

Authors

  • M PASTOR-ANGLADA
  • A FELIPE
  • F. CASADO
  • B. DEL SANTO
  • M. GÓMEZ-ANGELATS
  • A. FERRER-MARTÍNEZ

Keywords:

Nucleoside, Transport, SPNT, cNTl, Liver, Hepatoma, Fetus

Abstract

Nucleoside uptake into rat hepatocytes is mediated by, at least, three independent

transport systems, a Na+-dependent purine-preferring system (NI-like), a Na+-dependent

pyrimidine-preferring transport system (N2-like) and, finally, a Na+-independent agency

which is NBTI-insensitive. The concentrative component oftransport is highly regulated,

since it is induced in liver hypertrophia and hyperplasia and up-regulated by pancreatic

hormones. Two different cDNAs, SPNT and cNTl , apparently related to NI and N2

transport activities respectively, have been characterized so far in liver. The SPNT

mRNA is more abundant than the cNTI mRNA, but the biological activities associated

with NI and N2 transport systems are rather equivalent. Transformed liver parenchymal

cells (hepatoma cell lines) and fetal hepatocytes show a different pattem of nucleoside

transport systems. These highly proliferative cells show high nucleoside transport

activity although this is mediated mostly by a Na+-independent transport component

which is mostly sensitive to NBTI inhibition. This equilibrative NBTI-sensitive transport

system is not detected in adult hepatocytes. We conclude that the translocation of

nucleosides across the plasma membrane of the hepatocyte involves a complex combination

of carrier proteins which may differ depending on the differentiated state of the cell

rather than by their proliferative status.

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References

McGIVAN, J.D., PASTOR-ANGLADA, M. : Biochem.J. (1994), 299: 321-334.
BUSSOLATI, O., UGGERl, J., BELLETTI, S., DALL'ASTA, V., GAZZOLA, G. C.: FASEB J (1996), 10: 920-926.
SOLER, C., FELIPE, A. , CASADO, F. J., McGIVAN, J. D., PASTOR-ANGLADA, M.: Biochem J (1993), 289: 653-658 .
RUIZ-MONTASELL, B., GÓMEZ-ANGELATS, M., CASADO, F. J., FELIPE, A., McGIVAN, J. D., PASTOR-ANGLADA, M.: Proc Natl Acad Sci USA (1994) 91: 9569•9573.
MARTÍNEZ-MAS, J. V., CASADO, J., FELIPE, A., MARiN, J. J. G, PASTOR ANGLADA, M.: Biochem J (1993), 293 : 819-824.
AMAT, M. , FELIPE, A. , CASADO, J., PASTOR-ANGLADA, M.: Pediatr Res (1995 ), 38: 81-85.

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Published

1996-12-20

How to Cite

1.
PASTOR-ANGLADA M, FELIPE A, CASADO F, DEL SANTO B, GÓMEZ-ANGELATS M, FERRER-MARTÍNEZ A. Nucleoside transport systems in liver parenchymal cells. Isoform expression pattern in hepatocyte differentiation. Ars Pharm [Internet]. 1996 Dec. 20 [cited 2024 Dec. 22];37(4):791-806. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21298

Issue

Vol. 37 No. 4 (1996)

Section

Original Articles

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