Nucleoside transport systems in liver parenchymal cells. Isoform expression pattern in hepatocyte differentiation

Authors

  • M PASTOR-ANGLADA
  • A FELIPE
  • F. CASADO
  • B. DEL SANTO
  • M. GÓMEZ-ANGELATS
  • A. FERRER-MARTÍNEZ

Keywords:

Nucleoside, Transport, SPNT, cNTl, Liver, Hepatoma, Fetus

Abstract

Nucleoside uptake into rat hepatocytes is mediated by, at least, three independent

transport systems, a Na+-dependent purine-preferring system (NI-like), a Na+-dependent

pyrimidine-preferring transport system (N2-like) and, finally, a Na+-independent agency

which is NBTI-insensitive. The concentrative component oftransport is highly regulated,

since it is induced in liver hypertrophia and hyperplasia and up-regulated by pancreatic

hormones. Two different cDNAs, SPNT and cNTl , apparently related to NI and N2

transport activities respectively, have been characterized so far in liver. The SPNT

mRNA is more abundant than the cNTI mRNA, but the biological activities associated

with NI and N2 transport systems are rather equivalent. Transformed liver parenchymal

cells (hepatoma cell lines) and fetal hepatocytes show a different pattem of nucleoside

transport systems. These highly proliferative cells show high nucleoside transport

activity although this is mediated mostly by a Na+-independent transport component

which is mostly sensitive to NBTI inhibition. This equilibrative NBTI-sensitive transport

system is not detected in adult hepatocytes. We conclude that the translocation of

nucleosides across the plasma membrane of the hepatocyte involves a complex combination

of carrier proteins which may differ depending on the differentiated state of the cell

rather than by their proliferative status.

Downloads

Download data is not yet available.

References

McGIVAN, J.D., PASTOR-ANGLADA, M. : Biochem.J. (1994), 299: 321-334.
BUSSOLATI, O., UGGERl, J., BELLETTI, S., DALL'ASTA, V., GAZZOLA, G. C.: FASEB J (1996), 10: 920-926.
SOLER, C., FELIPE, A. , CASADO, F. J., McGIVAN, J. D., PASTOR-ANGLADA, M.: Biochem J (1993), 289: 653-658 .
RUIZ-MONTASELL, B., GÓMEZ-ANGELATS, M., CASADO, F. J., FELIPE, A., McGIVAN, J. D., PASTOR-ANGLADA, M.: Proc Natl Acad Sci USA (1994) 91: 9569•9573.
MARTÍNEZ-MAS, J. V., CASADO, J., FELIPE, A., MARiN, J. J. G, PASTOR ANGLADA, M.: Biochem J (1993), 293 : 819-824.
AMAT, M. , FELIPE, A. , CASADO, J., PASTOR-ANGLADA, M.: Pediatr Res (1995 ), 38: 81-85.

Downloads

Published

1996-12-20

How to Cite

1.
PASTOR-ANGLADA M, FELIPE A, CASADO F, DEL SANTO B, GÓMEZ-ANGELATS M, FERRER-MARTÍNEZ A. Nucleoside transport systems in liver parenchymal cells. Isoform expression pattern in hepatocyte differentiation. Ars Pharm [Internet]. 1996 Dec. 20 [cited 2024 May 19];37(4):791-806. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21298

Issue

Vol. 37 No. 4 (1996)

Section

Original Articles

License

The articles, which are published in this journal, are subject to the following terms in relation to the rights of patrimonial or exploitation:

  1. The authors will keep their copyright and guarantee to the journal the right of first publication of their work, which will be distributed with a Creative Commons BY-NC-SA 4.0 license that allows third parties to reuse the work whenever its author, quote the original source and do not make commercial use of it.

b. The authors may adopt other non-exclusive licensing agreements for the distribution of the published version of the work (e.g., deposit it in an institutional telematic file or publish it in a monographic volume) provided that the original source of its publication is indicated.

c. Authors are allowed and advised to disseminate their work through the Internet (e.g. in institutional repositories or on their website) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).