Effect of oleic acid on gap junction cornrnunication in rat astrocytes during developrnent

Authors

  • E. LAVADO
  • L. SÁNCHEZ-ABARCA
  • A. TABERNERO
  • J.P. BOLAÑOS
  • J.M. MEDINA

Keywords:

Lucifer yellow, Astrocytes, Glucose uptake, Glial cells, Intercellular communication

Abstract

The role of oleic acid in the modulation of gap junction permeability was studied

in cultured rat astrocytes by the scrape-Ioading/Lucifer yellow transfer technique.

lncubation with oleic acid caused a dose-dependent inhibition of gap junction permeability

by 79.5% at 50 IlM and no further inhibition was observed by increasing the oleic acid

concentration up to 100 1lM. The oleic acid-mediated inhibition of gap junction permeability

was reversible and was prevented by bovine serum albumin (BSA). The potency of

oleic acid-related compounds in inhibiting gap junction permeability was arachidonic

acid > oleic acid > oleyl alcohol > palmitoleic acid > stearic acid > octanol > caprylic

acid > palmitic acid > methyl-oleyl estero Oleic acid and arachidonic acid, but not

methyl-oleyl ester, increased glucose uptake by astrocytes. Our results support the

hypothesis that the inhibition of gap junction permeability is associated with the

increase in glucose uptake. We suggest that iduring the perinatal period, oleic acid may

be a physiological mediator in astrocyte differentiation by regulating intercellular

communication.

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References

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Published

1997-12-20

How to Cite

1.
LAVADO E, SÁNCHEZ-ABARCA L, TABERNERO A, BOLAÑOS J, MEDINA J. Effect of oleic acid on gap junction cornrnunication in rat astrocytes during developrnent. Ars Pharm [Internet]. 1997 Dec. 20 [cited 2024 May 19];37(4):739-51. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21281

Issue

Vol. 37 No. 4 (1996)

Section

Original Articles

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