Analysis of leishmania infection in mouse peritoneal macrophages

Authors

  • G. CARRILLO
  • G. MORALES
  • C. ALONSO

Keywords:

Leishmanolysin, Gp63, kinetoplastids, Leishmaniasis, Infection

Abstract

Mononuclear phagocytes are target cells for parasites of the genus Leishmania. From the entry into a vertebrate host until establishment of the infection, several crucial phases can be distinguished. These phases may represent the crucial point in the successful establishing of a Leishmania infection. The interaction between mononuclear phagocytes and the parasite plays a central role and may constitute a breakpoint in the infection process. One of the surface molecules of these parasites, gp63, a glycoprotein, has been described as ligand for attachment to host cell receptors. In order to inhibit the infection, polyclonal .antibodies against gp63 and its fragments as well as specific peptídes were used to block the interaction with host cell receptors. In both cases, either with peptides or with antibodies, significant inhibition ranging from 30-65% was obtained. The kinetic of the inhibition with different polyclonal antibodies suggests that gp63 must contain more than one single binding site.

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References

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Published

1997-03-20

How to Cite

1.
CARRILLO G, MORALES G, ALONSO C. Analysis of leishmania infection in mouse peritoneal macrophages . Ars Pharm [Internet]. 1997 Mar. 20 [cited 2024 Jul. 22];38(2-3):259-71. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21160

Issue

Vol. 38 No. 2-3 (1997)

Section

Original Articles

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