Molecular Characterization of a non-LTR retrotransposon. Genomic organization and model for integration

Authors

  • M. OLIVARES
  • F. MARTÍN
  • C. MARAÑÓN
  • M.C. López
  • A. López

Abstract

L1Tc is a non-L TR retrotransposon, dispersed throughout the genome of Trypanosoma cruzi, actively transcribed into poly (A)+ RNA. This element has at its 3' end a fragment of a highly repetitive sequence E 12 and at its 5'end a ribosomal mobile element-like sequence.

A genomic regíon of T. cruzi CL strain containing 3 copies of LI Tc, tandemly organized, has been characterized. Upstream of the genomic region containing the three

copies we found repeated sequences as E13 and DGF-l. A genomic region with the same organization and sequences, but lacking LI Tc, was found in the Maracay strain.

The deduced aminoacid sequence ofORFl ofLlTc exhibíts a significant homology with sequences of the AP endonuclease protein family. The analysis of the activity of the 40Kd recombinant protein, named NLlTc, obtained from the expression of the LlTc ORFI in a E. coli in vitro expression system, revealed that the sequence codes for a protein with endonuclease activity. Date are also presented showing that in vivo expression ofNLITc protein conferred viability by complementation to E. coli exonuclease III deletion mutants (BW286 strain). We propose that NLlTc generates free 3'OH sites in the chromosomal DNA necessary for LlTc retrotransposition.

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Published

1997-03-20

How to Cite

1.
OLIVARES M, MARTÍN F, MARAÑÓN C, López M, López A. Molecular Characterization of a non-LTR retrotransposon. Genomic organization and model for integration . Ars Pharm [Internet]. 1997 Mar. 20 [cited 2024 Jul. 22];38(2-3):209-20. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21142

Issue

Vol. 38 No. 2-3 (1997)

Section

Original Articles

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