Application Enzymatic Method for Analysis Vaginal Tabletas “Fluomizin”
DOI:
https://doi.org/10.30827/ars.v62i3.16866Keywords:
Dequalinium chloride, Fluomizin, enzymatic-photometric methodAbstract
Introduction: Development of a new enzymatic kinetic-photometric method for analysis of Dequalinium chloride, based on the use of enzymatic hydrolysis of acetylcholine, for determine the amount of Dequalinium chloride in the vaginal tablets “Fluomizin” No 6.
Method: The amount of Dequalinium chloride (DCh) was determined by the degree of inhibition of the enzymatic reaction, which was evaluated by the residual unreacted substrate - acetylcholine. Determination of the residual amount of acetylcholine in the reaction mixture was performed by a kinetic-photometric method using an indicator oxidation reaction of p-phenetidine with peracetic acid, which is formed during the auxiliary reaction of perhydrolysis with addition of excess hydrogen peroxide in the reaction mixture over a period of time.
Results: Optimal conditions were determined: pH 8.35 was selected, influence of nature of buffer solution, concentration of acetylcholine (0.05 mg / ml), cholinesterase (0.4 mg / ml), hydrogen peroxide (10,0%) and p-phenethedine (1 %), the incubation time (10 min).
Conclusions: The procedure for determination of Dequalinium chloride content in the vaginal tablets “Fluomizin” No 6 was developed. The kinetic parameters Km (Michaelis–Menten constant) and Vmax (maximal velocity) were determined.
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Khurasёva A. Otsenka effektyvnosty lechenyia bakteryalnoho vahynoza vahynalnimy tabletkamy s dekvalynyia khlorydom. Akusherstvo, hynekolohyia y reproduktsyia. 2018;12(3):29–34. doi.org/10.17749/2313-7347.2018.12.3.029-034
Dequalinium for bacterial vaginosis. Drug and Therapeutics Bulletin. 2017;55(5):54–57. http://dx.doi.org/10.1136/dtb.2017.5.0478
Mendling W., Weissenbacher E., Gerber S., Prasauskas V., Grob P. Use of locally delivered dequalinium chloride in the treatment of vaginal infections: a review. Archives of Gynecology and Obstetrics. 2015;293(3):469–484. doi.org/10.1007/s00404-015-3914-8
Derzhavnyi reiestr likarskykh zasobiv Ukrainy [http://www.drlz.kiev.ua].
Gagliardi L., Cavazzutti G., Tonelli D. Determination of Dequalinium Chloride and Related Impurities in Cosmetics and Pharmaceuticals by Reversed-Phase HPLC. Analytical Letters. 1998; 31(5): 829–839. doi.org/10.1080/00032719808002821
Taylor R., Toasaksiri S., Reid G., Wood D. Determination of the Quaternary Ammonium Compounds Dequalinium and Cetylpyridinium Chlorides in Candy-based Lozenges by High-performance Liquid Chromatography. The Analyst. 1997; 122(9):973–976. doi.org/10.1039/A703893C
Chen F.-A., Wu K.-S., Huang M.-C., Chen C.-Ya., Wu A.-B. Simultaneous quantitation of cationic disinfectants by high-performance liquid chromatography on a silica gel column using aqueous eluents. Journal of Food and Drug Analysis. 2001; 9(4):191–198.
Fang W.-H, Liu J., Shen J., Zhang L.-J. Determination of dequalinium chloride in dequalinium chloride buccal tablets by HPLC. Anhui Medical and Pharmaceutical Journa. 2011; l (7):837–839.
Mohamed H. A. Spectrophotometric Determination of Dequalinium Chloride. Analytical Letters.1993; 26(11):2421–2429. doi.org/10.1080/00032719308017481
Leung C. P., & Kwan S. Y. Spectrophotometric determination of dequalinium chloride in pharmaceutical preparations. The Analyst 1979; 104(1235): 143–146. doi.org/10.1039/an9790400143
Mohamed T. Y., Dossouki H. A., Moustafa M. M., Ghoname M. S. Spectrophotometric methods for the determination of dequalinium chloride and clidinium bromide using ion-pair complex formation with acid dyes. Egyptian Journal of Chemistry. 2008; 51(1):113–123.
Guilbault G. G., Sadar M. H. Use of enzymes in Analytical Chemistry. Analytical use of enzymes. Proceedings of the Analytical Division of the Chemical Society, 1977; 14(10):302–306. doi.org/10.1039/ad9771400302
Derzhavna farmakopeia Ukrainy (2015). Vols. 1–3. Vol. 1. 2nd ed. Derzhavne pidpryiemstvo «Ukrainskyi naukovyi ekspertnyi farmakopeinyi tsentr yakosti likarskykh zasobiv». Kharkiv, 1128.
Patent na korysnu model 117474 Ukraina, MPK G01N 33/68 (2006.01), G01 N 21/79(2006.01). Sposib vyznachennia aktyvnosti kholinesterazy krovi / Blazheievskyi M. Ye., Kovalska O. V., Diadchenko V. V. № u 2017 00721; zaiavl. 26. 01. 17; opubl. 26. 06. 17, Biul. №12. 4 s.
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