In vivo anti-inflammatory effect of saponin-enriched fraction from Agave brittoniana Trel subspecie brachypus
Keywords:
saponins, Agave, anti-inflammatory, carrageenan, granulomaAbstract
Introduction: Agave brittoniana Trel species. subsp. brachypus is characterized by its high content of steroidal saponins and these metabolites have anti-inflammatory properties. However, no previous studies indicate that extracts rich in saponins from this species have anti-inflammatory effect in vivo models.
Method: Steroidal saponins in n-butanolic extract were obtained from a hydroalcoholic extract of Agave brittoniana. Phytochemical studies were used to verify the saponins presence. Doses of 25, 50 and 100 mg/kg were used in the acute model of edema induced by carrageenan and in the chronic model of cotton pellet-induced granuloma. Five groups of six male Sprague-Dawley rats each were designed by each in vivo study: three groups for saponin-enriched fraction at different doses, a normal control group and a positive control, which was treated with indomethacin. The inhibition percentage of the inflammatory reaction was the major answer variable.
Results: The acute model of carrageenan-induced rat paw edema indicated that the groups corresponding to the doses of 50 and 100 mg/kg of saponin-enriched fraction showed the highest percentages of inhibition, after three hours of treatment. According to the chronic model results, saponins-enriched butanolic extract showed anti-inflammatory activity by reducing the dry weight of the granuloma and increasing the inhibition percentage.
Conclusions: Saponin-enriched fraction from Agave brittoniana T. subsp. brachypus, have an anti-inflammatory effect in acute and chronic experimental models.
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Calderón A, Karina M, Recio F. Revisión de las bases flsiopatológicas de la inflamación. Revista CONAMED. 2017;22(1):48-51.
Castilla M, de Lorenzo A, Martínez J. Respuesta inflamatoria sistemática: fisiopatologia y mediadores. Medicina Intensiva. 2000;24(8):353-60.
Jiménez G, Debesa F, Bastanzuri T, Pérez J, Ávila J. Comportamiento de las reacciones adversas a los analgésicos y antiinflamatorios no esteroideos notificadas por el Sistema Cubano de Farmacovigilancia en el 2001. Rev Cubana Farm. 2003;37(3):1-1.
Aravinthan A, Kim J, Antonisamy P, Kang C, Choi J, Kim N, et al. Ginseng total saponin attenuates myocardial injury via anti-oxidative and anti-inflammatory properties. J Ginseng Res. 2015;39 206-12.
Ramalingam M, Kim S. Pharmacological Activities and Applications of Spicatoside A. Biomol Ther. 2016;24(5):469-74.
Mohammed M, Osman W, Garelnabi E, Osman Z, Osman B, Khalid H, et al. Secondary metabolites as anti-inflammatory agents. Phytopharm. 2014;3(4):275-85.
Lande A, Ambavade S, Swami U, Adkar P, Ambavade P, Waghamare A. Saponins isolated from roots of Chlorophytumborivilianum reduce acute and chronic inflammation and histone deacetylase. J Integr Med. 2015 13(1):25-33.
Greuter W, Rankin-Rodríguez R. Plantas Vasculares de Cuba.
Inventario preliminar. Segunda edición, actualizada, de Espermatófitos
de Cuba con inclusión de los Pteridófitos: Botanischer
Garten & Botanisches Museum Berlin. Jardín Botánico
Nacional, Universidad de La Habana; 2017.
Monterrosas-Brisson N, Arenas M, Jiménez-Ferrer E, Jiménez-Aparicio A, Zamilpa A, Gonzalez-Cortazar, et al. Anti-Inflammatory activity of different Agave plants and the compound cantalasaponin-1. Molecules. 2013;18:8136-46.
Dunder R, Quaglio A, Maciel R, Luiz-Ferreira A, Almeida A, Takayama C, et al. Antiinflammatory and analgesic potential of hydrolyzed extract of Agave sisalana Perrine ex Engelm., Asparagaceae. Rev Bras Farmacogn. 2010;20:376-81.
Hernández-Valle E, Herrera-Ruiz M, Rosas G, Zamilpa A, Arenas M, Jiménez A, et al. Anti-Inflammatory effect of 3-O-[(6'-O-palmitoyl)-β-Dglucopyranosyl sitosterol] from Agave angustifolia on ear edema in mice. Molecules. 2014;19:15624-37.
da Silva B, Parente J. A New Bioactive Steroidal Saponin from Agave brittoniana. Z Naturforsch. 2007;62b: 1193 - 8.
Macías F, Guerra J, Simonet A, Nogueiras C. Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave britto¬niana Trel. Spp. Brachypus. Magn Reson Chem. 2007; 45: 615- 620
Norma Ramal de Salud Pública 309. Métodos de Ensayo de Droga cruda. MINSAP. 1991:30-40.
Guerra JO. Compuestos con actividad antiparasitaria, del “Agave brittoniana T’’ [Memoria en opción al grado de Doctor en Ciencias Químicas]. Dialnet: Universidad de Cádiz. Universidad de La Habana Universidad Central “Marta Abreu” de Las Villas; 2005. Available from: https://dialnet.unirioja. es/servlet/autor?codigo=4023972
Guerra J, Meneses A, Simonet A, Macías F, Nogueiras C, Gómez A, et al. Saponinas esteroidales de la planta Agave brittoniana (Agavaceae) con actividad contra el parásito Trichomona vaginalis. Rev Biol Trop. 2008;56(4):1645-52.
Macías F, Guerra J, Simonet A, Pérez A, Nogueiras C. Characterization of three saponins from a fraction using 1D DOSY as a solvent signal suppression tool. Agabrittonosides E–F. Furostane Saponins from Agave brittoniana Trel. spp. brachypus. Magn Reson Chem. 2010; 48:350-5.
Miranda M, Cuéllar A. Farmacognosia y Productos Naturales. 2a ed. La Habana: Editorial Félix Varela; 2001. 110 p.
CYTED. Manual de técnicas de investigación. Proyecto X-I. Búsqueda de principios bioactivos en Plantas de la Región. Subprograma X. Química Fina Farmacéutica. Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo. 1995:81-98.
Mahat M, Patil B. Evaluation of antiinflammatory activity of methanol extract of Phyllanthus amarus in experimental animal models. Indian J Pharm Sci. 2007;69(1):33-36
Sanmugapriya E, Shanmugasundara P, Venkataraman S. Antiinflammatory activity of Justicia prostrata Gamble in acute and sub-acute models of inflammation. Inflammopharmacology. 2005;13(5-6):493-500.
National Institutes of Health. Principles of laboratory animal care. NIH publication. 1985 p.85-23.
Quillay Davila MA, Arana Arias YAA, Jaramillo Jaramillo CG, Cuenca Buele S, Rojas de Astudillo LL, Jaramillo Alcívar V. Contenido de saponinas y actividad cicatrizante de Cecropia peltata y Parthenium hysterophorus. Rev Cubana Farm. 2018;51(3). Disponible en: http://www.revfarmacia.sld.cu/ index.php/far/article/view/250/147
Galindo J, González N, Luiz Abdalla A, Alberto M, Lucas RC, Dos Santos KC, et al. Effect of a raw saponin extract on ruminal microbial population and in vitro methane production with star grass (Cynodon nlemfuensis) substrate. Rev Cubana Ciencia Agrícola. 2016;50(1):77-88.
Tenon M, Feuillère N, Roller M, Birtić S. Rapid, cost-effective and accurate quantification of Yucca schidigera Roezl. steroidal saponins using HPLC-ELSD method. Food Chem. 2017;221:1245-52.
Adcock I, Ito K. Molecular mechanisms of corticosteroid actions. Monaldi Arch Chest Dis. 2000;55(3):256-66.
Ramamoorthy S, Cidlowski J. Corticosteroids: mechanisms of action in health and disease. Rheumatic Disease Clinics. 2016;42(1):15-31.
Sugishita E, Amagaya S, Ogihara Y. Anti-inflammatory testing methods: comparative evaluation of mice and rats. J Pharmaco-Biodyn. 1981;4: 565-75.
Mansouri M, Hemmati A, Naghizadeh B, Mard S, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol. 2015;47(3):292.
Sandhya S, Venkatramana K, Vinod KR, Chaitanya RK, Chandrasekhar J, Sudhakar K, et al. Membrane stabilizing potency of two Tephrosia species. J. Phytol. 2010;2(6),42-46.
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