Design and development of a facial Serum with <em>Lactobacillus fermentum</em> CECT 5716

Florina Iulia-Burra; Elena Ortega Martínez; Mª Adolfina Ruiz Martínez; Mª Encarnación Morales Hernández

doi:10.30827/ars.v64i3.27306

Autores/as

Florina Iulia-Burra Universidad de Granada, Facultad de Farmacia, Departamento de Farmacia y Tecnología Farmacéutica, Campus de Cartuja, Granada, 18071, España. https://orcid.org/0000-0002-4598-8062
Elena Ortega Martínez Universidad de Granada, Facultad de Farmacia, Departamento de Farmacia y Tecnología Farmacéutica, Campus de Cartuja, Granada, 18071, España. https://orcid.org/0000-0002-5406-5489
Mª Adolfina Ruiz Martínez Universidad de Granada, Facultad de Farmacia, Departamento de Farmacia y Tecnología Farmacéutica, Campus de Cartuja, Granada, 18071, España. https://orcid.org/0000-0002-9891-8578
Mª Encarnación Morales Hernández Universidad de Granada, Facultad de Farmacia, Departamento de Farmacia y Tecnología Farmacéutica, Campus de Cartuja, Granada, 18071, España. https://orcid.org/0000-0001-7701-6906

DOI:

https://doi.org/10.30827/ars.v64i3.27306

Palabras clave:

Lactobacillus fermentum, Microbiología, Xerosis, Fisiología/Estructura cutánea, Formulación/estabilidad, Sérum

Resumen

Introducción: De todos los órganos del cuerpo, la piel es el que está directa y constantemente expuesto a agentes externos. De ahí la importancia de proporcionarle todos los nutrientes necesarios para mantenerla sana, cuidada e hidratada. Con esta intención se ha diseñado y desarrollado un sérum hidratante con ingredientes activos naturales que incluye una cepa de la especie probiótica Lactobacillus fermentum CECT 5716, como innovación, para potenciar el efecto sinérgico del producto.

Método: El producto citado ha sido caracterizado mediante determinaciones de pH, conductividad y viscosidad para probar su estabilidad. Además, se ha estudiado la viabilidad del probiótico y la percepción de los consumidores en estudios de eficacia del producto.

Resultados: Los datos extraídos de las mediciones de pH y conductividad aseguran que la aplicación tópica del sérum es segura, al igual que la percepción de eficacia del producto por los consumidores, ofreciendo una mejor apariencia en términos de suavidad y luminosidad. Además, esta investigación ha revelado las características pseudoplásticas del sérum, óptimas para su propósito. Por último, se ha visto que la viabilidad probiótica disminuye con el tiempo.

Conclusiones: En base a los resultados obtenidos, se puede afirmar que nuestro sérum hidratante es seguro y eficaz en la hidratación cutánea, debido a las propiedades de las materias primas incorporadas y el mecanismo competitivo de los probióticos incluidos.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Sfriso R, Egert M, Gempeler M, Voegeli R, Campiche R. Revealing the secret life of skin - with the microbiome you never walk alone. Int J Cosmet Sci. 2020;42(2):116–26. Doi: 10.1111/ics.12594

Moysidis M, Stavrou G, ChevaIoanna A, Deka I A, Tsetis JK, Birba V, Kapoukranidou D, Tsaousi G, Kotzampassi K. The 3-D configuration of excisional skin wound healing after topical probiotic application. Injury Clin Plast Surg. 2022;531385–1393. Doi: 10.1016/j.injury.2022.02.006

Patti JM, Allen BL, McGavin MJ, Höök M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol. 1994;48: 585-617. Doi:10.1146/annurev.mi.48.100194.003101

Donlan RM, Costerton JW. Bioﬁlms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15: 27. Doi: 10.1128/CMR.15.2.167-193.2002

Chopra A, Gupta A. Skin as an immune organ and the site of biomimetic, non-invasive vaccination. Medicine in Novel Technology and Devices. 2022;16: 100196 . Doi:10.1016/j.medntd.2022.100196

Breton L, Jourdain R, Gueniche A, Bureau-Franz I, Fleith M, Malnoe A. Cosmetic and/or dermatological composition for prevention and/or treatment of sensitive or dry skin. US20090232785A1, 2009. https://patents.google.com/patent/US20090232785/en?oq=COSMETIC+AND%2fOR+DERMATOLOGICAL+COMPOSITION+FOR+PREVENTION+AND%2fOR+TREATMENT+OF+SENSITIVE+OR+DRY+SKIN

Musthaq S, Mazuy A, Jakus J. The microbiome in dermatology. Clin Dermatol. 2018;36(3): 390–8. Doi: 10.1016/j.clindermatol.2018.03.012

Bustamante M, Oomah BD, Oliveira WP, Burgos-Díaz C, Rubilar M, Shene C. Probiotics and prebiotics potential for the care of skin, female urogenital tract, and respiratory tract. Folia Microbiol. 2020;65(2): 245–64. Doi: 10.1007/s12223-019-00759-3

Gueniche A, Benyacoub J, Blum S, Breton L, Castiel I. Probiotics for skin benefits. In: Nutritional Cosmetics. Elsevier; 2009. Doi: 10.1016/B978-0-8155-2029-0.50029-6

Pérez-Sánchez A, Barrajón-Catalán E, Herranz-López M, Micol V. Nutraceuticals for skin care: a comprehensive review of human clinical studies. Nutrients. 2018;24;10(4):403. Doi: 10.3390/nu10040403

Lee HJ, Jeong SE, Lee S, Kim S, Han H, Jeon CO. Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels. Microbiologyopen. 2018;7(2): e00557. Doi:10.1002/mbo3.557

Knackstedt R, Knackstedt T, Gatherwright J. The role of topical probiotics in skin conditions: a systematic review of animal and human studies and implications for future therapies. Exp Dermatol. 2020;29(1): 15–21. Doi: 10.1111/exd.14032

Puebla-Barragan S, Reid G. Probiotics in cosmetic and personal care products: trends and challenges. Molecules. 2021;26;26(5):1249. Doi: 10.3390/molecules26051249

Al-Ghazzewi FH, Tester RF. Impact of prebiotics and probiotics on skin health. Benef Microbes. 2014;1;5(2): 99–107. Doi: 10.3920/BM2013.0040

Jung YO, Jeong H, Cho Y, Lee EO, Jang HW, Kim J, et al. Lysates of a probiotic, Lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model. IJMS. 2019;2;20(17): 4289. Doi: 10.3390/ijms20174289

Lee GR, Maarouf M, Hendricks AJ, Lee DE, Shi VY. Topical probiotics: the unknowns behind their rising popularity. Dermatol Online J. 2019;15;25(5):13030/qt2v83r5wk.

Cinque B, La Torre C, Melchiorre E, Marchesani G, Zoccali G, Palumbo P, et al. Use of Probiotics for Dermal Applications. In: Liong MT, editor. Probiotics. Berlin, Heidelberg: Springer Berlin Heidelberg; 201. Doi: 10.1007/978-3-642-20838-6_9

Pistone D, Meroni G, Panelli S, D’Auria E, Acunzo M, Pasala AR, et al. A Journey on the Skin Microbiome: Pitfalls and Opportunities. IJMS. 2021;12;22(18):9846. Doi:10.3390/ijms22189846

Tavaria FK. Topical use of probiotics: The natural balance: Porto Biomed J. 2017;2(3):69–70. Doi: 10.1016/j.pbj.2017.02.003

Draelos ZD. The science behind skin care: moisturizers. J Cosmet Dermatol. 2018;17(2):138-144. Doi:10.1111/jocd.12490

Szöllősi AG, Gueniche A, Jammayrac O, Szabó-Papp J, Blanchard C, Vasas N, et al. Bifidobacterium longum extract exerts pro-differentiating effects on human epidermal keratinocytes, in vitro. Exp Dermatol. 2017;26(1):92-4. Doi:10.1111/exd.13130

Butler É, Lundqvist C, Axelsson J. Lactobacillus reuteri DSM 17938 as a novel topical cosmetic ingredient: a proof of concept clinical study in adults with atopic dermatitis. Microorganisms. 2020;11;8(7):1026. Doi:10.3390/microorganisms8071026

Goik U, Goik T, Załęska I. The properties and application of argan oil in Cosmetology. Eur J Lipid Sci Technol. 2019;121(4):1800313. Doi:10.1002/ejlt.201800313

Lin TK, Zhong L, Santiago J. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. Int J Mol Sci. 2017;27;19(1):70. Doi:10.3390/ijms19010070

Kiralan M, Yildirim G. Rosehip (Rosa canina L.) Oil. In: Ramadan MF, editor. Fruit Oils: Chemistry and Functionality [Internet]. Cham: Springer International Publishing; 2019. p. 803–14. Doi: 10.1007/978-3-030-12473-1_43

Lochhead R.Y. The Use of Polymers in Cosmetic Products. In: Cosmetic Science and Technology. Elsevier, 2017, 171-221. Doi: 10.1016/B978-0-12-802005-0.00013-6.

American Pharmaceutical Association, Sheskey PJ, editors. Handbook of pharmaceutical excipients. 8. ed. London: Pharmaceutical Press; 2017.

Yu C. The use of corn-derived ingredients in personal care applications. In: Dayan N, editor. Handbook of Formulating Dermal Applications. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2016. Doi: 10.1002/9781119364221.ch5

Abatangelo G, Vindigni V, Avruscio G, Pandis L, Brun P. Hyaluronic acid: redefining its role. Cells. 2020;21;9(7):1743. Doi:10.3390/cells9071743

Fowler J. Understanding the role of Natural Moisturizing Factor in skin hydration. Feature story. 2012 [cited 20 January 2023]. Available in: https://practicaldermatology.com/articles/2012-jul/understanding-the-role-of-natural-moisturizing-factor-in-skin-hydration

Surini S, Nursatyani K, Ramadon D. Gel formulation containing microcapsules of grape seed oil (Vitis vinifera L.) for skin moisturizer. J Young Pharm. 2018;15;10(1):41–7. Doi:10.5530/jyp.2018.10.11

Aslam MN, Lansky EP, Varani J. Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol. 2006;103(3): 311–8. Doi: 10.1016/j.jep.2005.07.027

Aldag C, Nogueira Teixeira D, Leventhal PS. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature. Clin Cosmet Investig Dermatol. 2016; 9: 411–9. Doi:10.2147/CCID.S116158

Ramanunny AK, Wadhwa S, Gulati M, Singh SK, Kapoor B, Dureja H, et al. Nanocarriers for treatment of dermatological diseases: principle, perspective and practices. Eur J Pharmacol. 2021;890:173691. Doi:10.1016/j.ejphar.2020.173691

Thermo Scientific HAAKE RotoVisco 1 [Internet]. Rheology Solutions. Available from: http://www.rheologysolutions.com/thermo-scientific-haake-rotovisco-1/

Thermo Scientific HAAKE RheoWin Software [Internet]. Rheology Solutions. Available from: http://www.rheologysolutions.com/thermo-scientific-haake-rheowin-software/

Conductímetro EC-Metro BASIC 30 [Internet]. Crison Instruments. Available from: http://www.crisoninstruments.com/es/laboratorio/conductimetro/de-sobremesa/ec-metro-basic-30

Tsai CC, Chan CF, Huang WY, Lin JS, Chan P, Liu HY, et al. Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. Molecules. 2013;15;18(11):14161–71. Doi:10.3390/molecules181114161

Bond DM, Morris JM, Nassar N. Study protocol: evaluation of the probiotic Lactobacillus fermentum CECT5716 for the prevention of mastitis in breastfeeding women: a randomised controlled trial. BMC Pregnancy Childbirth. 2017;17(1):148. doi:10.1186/s12884-017-1330-8

Rodríguez-Sojo MJ, Ruiz-Malagón AJ, Rodríguez-Cabezas ME, Gálvez J, Rodríguez-Nogales A. Limos Lactobacillus fermentum CECT5716: mechanisms and therapeutic insights. Nutrients. 2021;21;13(3):1016. Doi: 10.3390/nu13031016

Zhao Y, Hong K, Zhao J, Zhang H, Zhai Q, Chen W. Lactobacillus fermentum and its potential immunomodulatory properties. J Functional Foods. 2019;56: 21–32. Doi: 10.3389/fmicb.2022.1073922

Monografía LACTANZA Hereditum®. 2014. https://www.lactanciasindolor.com/probioticos-mamarios/lactanza-hereditum/

Universitat Politècnica de València E. Modelo experimental para estimar la viscosidad de fluidos no newtonianos: ajuste a expresiones matemáticas convencionales. Ing agua. 2014; 29:18(1):ix.

Adewale FJ, Lucky AP, Oluwabunmi AP, Boluwaji EF. Selecting the most appropriate model for rheological characterization of synthetic based drilling mud. Int J Applied Engin Res. 2017;12(18):16.

Knowlton J, Pearce S. Rheology of cosmetics systems. In: Handbook of Cosmetic Science & Technology. Elsevier; 1993. Doi: 10.1016/B978-1-85617-197-7.50007-1

Gunaseelan K, Umlong IM, Mukhim T, Ismail K. Electrical conductance behavior of Oil-in-Water microemulsions stabilized by Sodium Dodecyl Sulfate and 1-Butanol. Langmuir. 2003;19(18):7276–81. Doi.org/10.1021/la034899k

Maier RM, Pepper IL. Bacterial growth. In: Environmental Microbiology. Elsevier; 2015. Doi: 10.1016/B978-0-12-394626-3.00003-X

Barer MR. Bacterial growth, culturability and viability. In: Molecular Medical Microbiology. Elsevier; 2015. Doi: 10.1016/B978-0-12-397169-2.00010-X