Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus

Diana C. Restrepo-Espinosa; Juliana María Motta; Felipe Castro O. de B. Teixeira; Yony Román; Jhonny Colorado-Ríos; Mauro S. G. Pavão; Alejandro Martínez

doi:10.30827/ars.v58i3.6526

Autores/as

Diana C. Restrepo-Espinosa Universidad de Antioquia
Juliana María Motta Universidade Federal do Rio de Janeiro
Felipe Castro O. de B. Teixeira Universidade Federal do Rio de Janeiro
Yony Román Universidade Federal do Paraná
Jhonny Colorado-Ríos Universidad de Antioquia
Mauro S. G. Pavão Universidade Federal do Rio de Janeiro
Alejandro Martínez Universidad de Antioquia

DOI:

https://doi.org/10.30827/ars.v58i3.6526

Palabras clave:

Anticoagulantes, ascidia, Microcosmus exasperatus, polisacáridos

Resumen

Objetivos: El efecto anticoagulante y la citotoxicidad de una fracción de polisacáridos de alto peso molecular (1000RS), obtenida de la túnica de la ascidia Microcosmus exasperatus, fueron evaluados.

Métodos: La actividad anticoagulante de 1000RS fue evaluada mediante los ensayos de tiempo de tromboplastina parcial activado (TTPa), tiempo de trombina (TT), tiempo de protrombina (TP), anti factor Xa y anticoagulante lúpico (dRVVT). La citotoxicidad sobre las células hematopoyéticas murinas fue evaluada usando el método del MTT.

Resultados: Esta fracción rica en galactosa mostró ser un anticoagulante potencial debido a su efecto inhibidor de la vía intrínseca de la coagulación. Así mismo, las dosis anticoagulantes de esta fracción no afectan la viabilidad celular, lo cual ratifica su potencial como agente terapéutico.

Conclusión: El efecto anticoagulante in vitro de 1000RS ocurre a dosis inocuas, sin embargo, éste debe ser evaluado en modelos in vivo, así como su citotoxicidad sobre células humanas normales.

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