Reverse transcriptase-like activity in the mobile element L1 tc

Authors

  • C.L GONZÁLEZ
  • M.C. THOMAS
  • F. MARTÍN
  • M.C. López

Keywords:

Trypanosoma cruzi, Polimerasa ARN-dependiente, Homopolímeros ARN., Inhibidores RT.

Abstract

By using synthetic homopolyrners and specific inhibitors the presence of a RT-Iike activity was revealed to be present in Trypanosoma cruzi, the parasite responsible for the Chagas' disease. We show, moreover, that this parasite has in its genome a non-LTR type mobile element, denominated L1Tc, and that the recombinant protein obtained from translation of the ORF2 of this element exhibits retrotranscriptase activity. The presence of a RT-líke activity in the nuclear fraction of the parasite, the fact that the L1Tc element was isolated as a cDNA and that the ORF2 derived protein has RT-like activity strongly suggest that L1Tc may well be responsible for the detected nuclear RT-like activity and that it functions as an active mobile element for retrotransposition.

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References

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Published

1997-03-20

How to Cite

1.
GONZÁLEZ C, THOMAS M, MARTÍN F, López M. Reverse transcriptase-like activity in the mobile element L1 tc . Ars Pharm [Internet]. 1997 Mar. 20 [cited 2024 May 19];38(2-3):221-7. Available from: https://revistaseug.ugr.es/index.php/ars/article/view/21150

Issue

Vol. 38 No. 2-3 (1997)

Section

Original Articles

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