Inhibitory effect of isolated Lactobacillus from oral cavity against bacterial Pathogens and its effect on health promotion

Khalil Azizian; Sepehr Taghizadeh; Rasoul Hosseinpour; Asghar Tanomand; Elham Sheikhsaran; Naser Alizadeh; Behrooz Naghili; Hossein Samadi Kafil

doi:10.30827/ars.v60i1.7943

Autores/as

Khalil Azizian Infectious and Tropical Diseases Research Center, Tabriz university of medical science, Tabriz, Iran. Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Liver and Gastrointestinal Diseases Research Center, Tabriz university of medical science, Tabriz, Iran.
Sepehr Taghizadeh Infectious and Tropical Diseases Research Center, Tabriz university of medical science, Tabriz, Iran
Rasoul Hosseinpour Liver and Gastrointestinal Diseases Research Center, Tabriz university of medical science, Tabriz, Iran.
Asghar Tanomand Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
Elham Sheikhsaran Infectious and Tropical Diseases Research Center, Tabriz university of medical science, Tabriz, Iran
Naser Alizadeh Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Behrooz Naghili Infectious and Tropical Diseases Research Center, Tabriz university of medical science, Tabriz, Iran
Hossein Samadi Kafil Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran

DOI:

https://doi.org/10.30827/ars.v60i1.7943

Palabras clave:

Lactobacillus, condición de salud, actividad antibacteriana, cavidad oral

Resumen

Objetivos: Determinar el efecto inhibidor del Lactobacillus aislado de la cavidad oral contra patógenos bacterianos e investigar la correlación entre la presencia de cepas de Lactobacillus y la promoción de la salud.

Método: se recolectaron cien muestras de saliva de la cavidad oral de consumidores de productos lácteos y se investigó el aislamiento e identificación de la cepa de Lactobacillus mediante cultivo convencional y secuenciación de 16SrRNA. Además, se realizó un ensayo de difusión en pocillos para determinar la actividad antibacteriana de las cepas de Lactobacillus contra patógenos bacterianos que incluyen Salmonella typhimurium, Klebsiella pneumoniae, Shigella sonnei, Shigella dysenteriae, Enterococcus faecalis y Enterococcus faecium. Finalmente, se investigó la asociación entre el estado de salud y el aislamiento de Lactobacillus y se obtuvieron los datos utilizando el cuestionario mediante la prueba de chi-cuadrado.

Resultados: Se aislaron treinta cepas de Lactobacillus de 100 muestras de saliva. La cepa aislada más común fue L. gasseri (n = 18), seguida por L. vaginalis (n = 3) y L. salivarius (n = 3). Todas las cepas de Lactobacillus demostraron actividad antibacteriana contra al menos uno de los patógenos investigados. Sin embargo, los resultados más fuertes fueron obtenidos por L. vaginalis contra K. pneumonia. No se encontraron correlación entre la presencia de algunas de las treinta cepas de Lactobacillus y la promoción de la salud. Sin embargo, solo la especie L. gasseri tuvo un impacto positivo significativo en la salud de sus hospedadores (P <0,05).

Conclusión: solo algunas especies de Lactobacillus tienen un impacto positivo en la promoción de la salud. A pesar de la actividad débil contra los patógenos investigados, L. gasseri tiene un impacto positivo en el problema mental (ira intensa y depresión) de sus huéspedes.

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